Inhibitory effects of matrine on the production of matrix metallo - proteinases of human gastric carcinoma cell line MKN - 45
- Received Date: 2006-01-15
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Key words:
- matrine /
- matrix melalloproteinases /
- gastric carcinoma /
- invasive
Abstract: Objective : To explore the mechanism of therapeutic effect of matrine on human gastric carcinoma by inhibiting the production of matrix metallo-proleinases of cell line MKN - 45 induced by it. Methods : The production of matrix metalloproteinases (MMP -2,MMP -9)of MKN -45 cells were measured by ELISA and gelatin zymogrmn. Results : In the dose of 5 -20μmol/L, matrine can significantly inhibit MMP-2 as well as MMP-9 expressions in MKN -45 cells. Especially, in the zymographic analysis of MMPs pxressions in conditional culture media, matrine 20μmol/L can greatly inhibit the MMP -2 and MMP -9 enzyme activity. Conclusion : Matrine could significantly inhibit the production and activity of MMPs in gastric carcinoma cell line MKN -45, which showed the inhibitory effect on the metastasis of gastric carcinoma cells.
Citation: | LU Zhen-dong, Zhang Ge. Inhibitory effects of matrine on the production of matrix metallo - proteinases of human gastric carcinoma cell line MKN - 45[J]. Journal of Pharmaceutical Practice and Service, 2006, (3): 147-149. |