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LIU Sheng-jie, WANG Li-na, DONG Sheng-hui. Determination of puerarin in puerariae radix dripping pills by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2006, (3): 164-166.
Citation: LIU Sheng-jie, WANG Li-na, DONG Sheng-hui. Determination of puerarin in puerariae radix dripping pills by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2006, (3): 164-166.

Determination of puerarin in puerariae radix dripping pills by HPLC

  • Received Date: 2006-04-05
  • Objective :To establish a HPLC method for determination of puerarin in radix puerariae dripping pills. Methods :The radix puerariae dripping pills were dissolved with 30% alcohol by ultrasonic wave. The puerarin was separated by Kromasil C18 analysis column. The mobile phase was composed of acetonltrile : water = 10 : 90( V/V). The flow rate of mobile phase was 1mL/min. The detection wavelength was UV 250nm. Results :There was a good linearity from 10 μg/mL to 200 μg/mL (r=0.999 8) in separation of puerarin. The average recovery was 100. 76% and the relative standard deviation 1. 12% (n=6). Conclusion :The method is simple and fast, and the results are reliale.
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通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Determination of puerarin in puerariae radix dripping pills by HPLC

Abstract: Objective :To establish a HPLC method for determination of puerarin in radix puerariae dripping pills. Methods :The radix puerariae dripping pills were dissolved with 30% alcohol by ultrasonic wave. The puerarin was separated by Kromasil C18 analysis column. The mobile phase was composed of acetonltrile : water = 10 : 90( V/V). The flow rate of mobile phase was 1mL/min. The detection wavelength was UV 250nm. Results :There was a good linearity from 10 μg/mL to 200 μg/mL (r=0.999 8) in separation of puerarin. The average recovery was 100. 76% and the relative standard deviation 1. 12% (n=6). Conclusion :The method is simple and fast, and the results are reliale.

LIU Sheng-jie, WANG Li-na, DONG Sheng-hui. Determination of puerarin in puerariae radix dripping pills by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2006, (3): 164-166.
Citation: LIU Sheng-jie, WANG Li-na, DONG Sheng-hui. Determination of puerarin in puerariae radix dripping pills by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2006, (3): 164-166.

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