Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 25 Issue 4
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2007  >  (4): 238-240

WANG Hui, ZHANG Guo-qing. Determination of ropivacaine in human plasma by reversed-phase high performance liquid chromatography[J]. Journal of Pharmaceutical Practice and Service, 2007, (4): 238-240.
Citation: WANG Hui, ZHANG Guo-qing. Determination of ropivacaine in human plasma by reversed-phase high performance liquid chromatography[J]. Journal of Pharmaceutical Practice and Service, 2007, (4): 238-240.
shu

Determination of ropivacaine in human plasma by reversed-phase high performance liquid chromatography

  • Eastern Hepatobiliary Surgery Hospital of Shanghai,Shanghai 200438,China

  • Received Date: 2006-03-23
  • Objective: To establish an accurate and rapid method for determination of ropivacaine by RP-HPLC. Methods: The sample was treated by the addition of bupivacaine as an internal standard,extraction with aether and n-hexane,and dryed by N2.The residue dissolved in the mobile phase and injected into chromatographic system.The HPLC system consisted of AgilentC18 column（4.6 mm×150 mm,5μm）and mobile phase of 0.045 mol/L KH2PO4（pH3.0）-methol（55:45）.Velocity of flow was 1.0 mL/min.The detection wavelength was 210 nm.Column temperature was 30℃. Results: The linear range was from 0.2509μg/mL to 5.018μg/mL with r of 0.999 6,the average recovery was 102.0%.RSD of Intra-day and Inter-day<15%.The results were accord with methodology. Conclusion: This method is quick and stable for ropivacaine monitoring in clinical research.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(2551) PDF downloads(28) Cited by()

Related
Proportional views

Determination of ropivacaine in human plasma by reversed-phase high performance liquid chromatography

  • Eastern Hepatobiliary Surgery Hospital of Shanghai,Shanghai 200438,China
  • Received Date: 2006-03-23

Abstract: Objective: To establish an accurate and rapid method for determination of ropivacaine by RP-HPLC. Methods: The sample was treated by the addition of bupivacaine as an internal standard,extraction with aether and n-hexane,and dryed by N2.The residue dissolved in the mobile phase and injected into chromatographic system.The HPLC system consisted of AgilentC18 column（4.6 mm×150 mm,5μm）and mobile phase of 0.045 mol/L KH2PO4（pH3.0）-methol（55:45）.Velocity of flow was 1.0 mL/min.The detection wavelength was 210 nm.Column temperature was 30℃. Results: The linear range was from 0.2509μg/mL to 5.018μg/mL with r of 0.999 6,the average recovery was 102.0%.RSD of Intra-day and Inter-day<15%.The results were accord with methodology. Conclusion: This method is quick and stable for ropivacaine monitoring in clinical research.

WANG Hui, ZHANG Guo-qing. Determination of ropivacaine in human plasma by reversed-phase high performance liquid chromatography[J]. Journal of Pharmaceutical Practice and Service, 2007, (4): 238-240.
Citation: WANG Hui, ZHANG Guo-qing. Determination of ropivacaine in human plasma by reversed-phase high performance liquid chromatography[J]. Journal of Pharmaceutical Practice and Service, 2007, (4): 238-240.
shu

    HTML

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return