Determination of chlorhexidine acetate in appliance-preserving solution by RP-HPLC
- Received Date: 2007-03-27
- Rev Recd Date: 2007-06-18
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Key words:
- chlorhexidine acetate /
- appliance preserving solution /
- RP-PLC /
- determination
Abstract: Objective: To develop a RP-HPLC method for determination of chlorhexidine acetate in appliance preserving solution. Methods: RP-HPLC was used for the determination of chlorhexidine acetate in appliance's preserving solution on a Shim-pack CLC-C8 column, with acetonitrile-water (including 1% triethylamine,adjecting pH to 4.0 by glacial acetate)(42-58)as mobile phase.The detection wavelength was 258 nm,flow rate was 1.0 mL/min, column temperature was 30 ℃,sample injection volume was 20 μL. Results: Chlorhexidine acetate had a good linear coefficient (r=0.999 8) over the range of 20-80 μg/mL.The average recoveries of chlorhexidine acetate was 99.96%,RSD=1.71% (n=6). Conclusion: This method was quick and accurate for determination of chlorhexidine acetate in appliance preserving solution.
Citation: | HUANG Jin-hua, MAO Gui-fu, WU Rong, LING Yi. Determination of chlorhexidine acetate in appliance-preserving solution by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (1): 48-50. |