Determination of bergeninum in Yinxian Tongfeibao granules by HPLC
- Received Date: 2006-04-24
- Rev Recd Date: 2006-08-28
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Key words:
- HPLC /
- Yinxian Tongfeibao /
- granules /
- bergeninum
Abstract: Objective: To develop a HPLC method for determining bergeninum in Yinxian Tongfeibao granules. Methods: Zorbax Eclipse XDB-C18 column was used and methanol-water-acetic acid glacial(18-81-1) was used as mobil phase,detection wavelegth was 275 nm,the flow rate was 0.8 mL/min,column temperature was 30 ℃. Results: There was a good linear relationship in the range of 0.43-2.15 μg, r=0.999 88.The average recovery was 98.33% with RSD=2.68%. Conclusion: The method is rapid ,accurate and repeatable.It can be applied to an effective control of the quantity of Yinxian Tongfeibao granules.
Citation: | SU Ming-wu, LIU Jun, YAO Xue-lian, YUAN Zheng, YIN Jiu. Determination of bergeninum in Yinxian Tongfeibao granules by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (1): 51-53. |