Simultaneous determination of chlorogenic acid and baicalin in Yinhuang oral liquid by HPLC
- Received Date: 2007-03-19
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Key words:
- HPLC /
- Yinhuang oral liquid /
- chlorgenic acid /
- baicalin
Abstract: Objective: To develop a HPLC quantitative method for the determination of chlorgenic acid and baicalin in Yinhuang Oral liquid. Methods: An Agilent C18 column(ZORBAX Eclipse XDB-C18,4.6×150 mm,5 μm)was used and a mixture of methanol-water-phosphorc acid(50:950:5)was used as mobile phase A,methanol as mobile phase B,gradient elution:0~30,A:95%~35%,B:5%~65%.The flow rate was 1.0 mL/min and monitored at 327 nm. Results: The retention time of chlorgenic acid and baicalin were 10.5 min and 23.8 min,resp...
Citation: | TAN Sheng-jian, LIU Gang, ZHANG Jie, LIU Chang-ye, PAN Min-xiang, CHANG Hua. Simultaneous determination of chlorogenic acid and baicalin in Yinhuang oral liquid by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (2): 138-140. |