Method for quality control of Cortex Phellocienciri in Niuhuang Yijin tablets

Key words: 
• Niuhuang Yijin tablets /
• berherine /
• HPLC

Abstract: Objective: To develop a method to determine the content of berberine hydrochlide in Niuhuang Yijin tablets to guarantee the quality of it. Methods: The determination was performed on Kromasil C₁₈ column(4.6×250 mm,5 μm) with the mobile phase of CH₃CN(0.05 mol/L)-KH₂PO₄(pH 3.0±0.05,0.025 mol/L)-sodium dodecylsulfonate.The detection wavelength was 348nm. Results: The correlation coefficients was 0.999 3 when the linear range was from 0.094 32 to 0.660 2 μg.The average recovery was 100.29% and RSD was 0.58%. Conclusion: The method was pertinence and accuracy which can be used for quality control of Niuhuang Yijin tablets.