Determination of cryptotanshinone and tanshinone ⅡA in Danshentong buccal tablets by HPLC
-
Key words:
- HPLC /
- Danshentong buccal tablets /
- cryptotanshinone /
- tanshinone ⅡA
Abstract: Objective: To develop a HPLC quantitative method for the determination of cryptotanshinone and tanshinone ⅡA in Danshentong buccal tablets. Methods: The chromatographic conditions include column C18(Agilent ZORBAX Eclipse XDB-C18,4.6×150 mm,5 μm),mobile phase:acetonitrile-water(55-45),flow rate 1.0 mL/min and detection wavelength 327 nm. Results: The regresstion equation of cryptotanshinone and tanshinone ⅡA was Y=0.000 270 9X+0.001 090,r=0.999 9,Y=0.000 255 6X+0.001 055,r=0.999 9.The linear range was 0.036 00~1.440 μg and 0.030 00~1.200 μg.The average recovery of cryptotanshinone and tanshinone ⅡA were 101.7%(RSD 0.28%),100.8%(RSD 0.50%)respectively. Conclusion: The method was sensitive,rapid and accurate.
Citation: | XUE Ke-chang, SHI Ning, TAN Sheng-jian, CHI Jia-ping, JIANG Li, WU Jiu-hong. Determination of cryptotanshinone and tanshinone ⅡA in Danshentong buccal tablets by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (2): 143-145. |