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ZHANG Jun, WANG Hui, JIN Xing. Characteristics of membrane current induced by excitory amino acid receptors and the acting way of L-SOP[J]. Journal of Pharmaceutical Practice and Service, 2008, (4): 258-260,296.
Citation: ZHANG Jun, WANG Hui, JIN Xing. Characteristics of membrane current induced by excitory amino acid receptors and the acting way of L-SOP[J]. Journal of Pharmaceutical Practice and Service, 2008, (4): 258-260,296.

Characteristics of membrane current induced by excitory amino acid receptors and the acting way of L-SOP

  • Received Date: 2008-07-08
  • Objective: To study the characters of membrane current of types of excitory amino acide and the acting way of TypeⅢmGluR(metabotropic glutamate receptor) agonist—L-SOP among them. Methods: A total receptor RNA was isolated from adult rat brain by acid guanidnium thiocyanate-phenol-chloroform(AGPC) method,mRNA was isolated from the total RNA by oligo(dT)-cellu- cose chromatography,and was then micro-injected into Xenopus oocytes to express,through a two-microeleetrode voltage clamp tech- nique.The ooeytes were refused with varieties of types of excitory amino acid. Results: 100μM KA(kainate) (ionic glutamate recep- tor) induced first a peak current and then a slowly descending current,which desentized at last.The mean maintained time was 37.57 min.2μM QA(quisqualate) (TYPEⅠmGluR) induced regulary calcium oscillation with a maintained time of (6.72±1.33) min. Both of the maintained time were shortened the agonist concentration increased.0.8 mM L-SOP of TYPEⅢmGluR agonist induced a kind of disciplinary oscillation with a maintained time of (20.17±8.47) min which was also shortened with an increasing concentra- tion,while the oscillation coule be antagonized by TYPEⅠmGluR antagonist—L-AP3. Conclusion:: Each of these three types of gluta- mate amino acid receptors has its speuniquecial current mode when irritated;L-SOP is not only an agonist of TYPEⅢmGluR,but also a weak agonist of TYPEⅠ,that is,its acting way of producing oscillation is through TYPEⅠreceptor.
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Characteristics of membrane current induced by excitory amino acid receptors and the acting way of L-SOP

Abstract: Objective: To study the characters of membrane current of types of excitory amino acide and the acting way of TypeⅢmGluR(metabotropic glutamate receptor) agonist—L-SOP among them. Methods: A total receptor RNA was isolated from adult rat brain by acid guanidnium thiocyanate-phenol-chloroform(AGPC) method,mRNA was isolated from the total RNA by oligo(dT)-cellu- cose chromatography,and was then micro-injected into Xenopus oocytes to express,through a two-microeleetrode voltage clamp tech- nique.The ooeytes were refused with varieties of types of excitory amino acid. Results: 100μM KA(kainate) (ionic glutamate recep- tor) induced first a peak current and then a slowly descending current,which desentized at last.The mean maintained time was 37.57 min.2μM QA(quisqualate) (TYPEⅠmGluR) induced regulary calcium oscillation with a maintained time of (6.72±1.33) min. Both of the maintained time were shortened the agonist concentration increased.0.8 mM L-SOP of TYPEⅢmGluR agonist induced a kind of disciplinary oscillation with a maintained time of (20.17±8.47) min which was also shortened with an increasing concentra- tion,while the oscillation coule be antagonized by TYPEⅠmGluR antagonist—L-AP3. Conclusion:: Each of these three types of gluta- mate amino acid receptors has its speuniquecial current mode when irritated;L-SOP is not only an agonist of TYPEⅢmGluR,but also a weak agonist of TYPEⅠ,that is,its acting way of producing oscillation is through TYPEⅠreceptor.

ZHANG Jun, WANG Hui, JIN Xing. Characteristics of membrane current induced by excitory amino acid receptors and the acting way of L-SOP[J]. Journal of Pharmaceutical Practice and Service, 2008, (4): 258-260,296.
Citation: ZHANG Jun, WANG Hui, JIN Xing. Characteristics of membrane current induced by excitory amino acid receptors and the acting way of L-SOP[J]. Journal of Pharmaceutical Practice and Service, 2008, (4): 258-260,296.

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