Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code

YAN Fei, ZHANG Qiao-yan, ZHANG Hong, HAN Ting, JIAO Lei, QIN Lu-ping. Synergistic protective effect of Fructus Schisandrae chinensis with Astragalus polysaccharides on paracetamol-induced acute liver injury in mice[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(5): 340-344.
Citation: TAN Sheng-jian, LIU Gang, WANG Hai-tao, ZHAO Miao, JIANG ren, ZHANG Hua. Determination of vitaminB6,phenobarbital,chlorphenamine maleate and diazepam in compound benxiaonamin tablets by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (4): 286-289.

Determination of vitaminB6,phenobarbital,chlorphenamine maleate and diazepam in compound benxiaonamin tablets by HPLC

  • Objective: To develop an HPLC quantitative method for the determination of vitaminB6,phenobarbital,chlorphena- mine maleate and diazepam in compound benxiaonamin tablets. Methods: The chromatographic conditions include column C18 (150 mm×4.6 mm,4.6μm);mobile phase A:aeetonitrile-water-triethylamine(100:900:1,contain 0.01 mol/L Sodium Heptane, adjusted to pH 3.5 by acetic aeid);mobile phase B:aeetonitrile,gradient elution:0~12 min,A:100%~70%,12~20 min,A: 70%.The flow rate was 1 mL/min and monitored at 260 nm. Results: The retention time of vitaminB6,phenobarbilal,chlorphena- mine maleate and diazepam were 4.6 min,10.2 min,12.3 min and 14.3 min respectively.The regress equation for VitaminB6 was y=251.4x+2.403,r=0.999 8,and the linear range was0.1684~2.021μg,phenobarbital y=145.8x+1.612,r=0.9999 and the linear range was 0.510 8~6.130μg;ehlorphenamine maleate y=697.3x-7.252,r=0.999 9,and the linear range was 0.122 8~1.474μg,diazepam y=2767x+17.75,r=0.999 8 and the linear range was 0.049 60~0.595 2μg.The average re- covery of vitaminB6,phenobarbital,chlorphenamine maleate and diazepam were 100.1%,99.4%,99.3% and 100.7%;and RSD were 0.18%,0.13%,0.47% and 0.16% respectively. Conclusion:: The method was sensiteve,time-saving and accurate.
  • Created with Highcharts 5.0.7Amount of accessChart context menuAbstract Views, HTML Views, PDF Downloads StatisticsAbstract ViewsHTML ViewsPDF Downloads2024-052024-062024-072024-082024-092024-102024-112024-122025-012025-022025-032025-0400.250.50.7511.25Highcharts.com
    Created with Highcharts 5.0.7Chart context menuAccess Class DistributionFULLTEXT: 91.2 %FULLTEXT: 91.2 %META: 7.3 %META: 7.3 %PDF: 1.5 %PDF: 1.5 %FULLTEXTMETAPDFHighcharts.com
    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 16.6 %其他: 16.6 %China: 1.3 %China: 1.3 %Indianapolis: 0.5 %Indianapolis: 0.5 %Korea Republic of: 0.3 %Korea Republic of: 0.3 %Taiwan, China: 0.3 %Taiwan, China: 0.3 %[]: 0.3 %[]: 0.3 %上海: 1.0 %上海: 1.0 %东莞: 0.3 %东莞: 0.3 %东营: 0.3 %东营: 0.3 %兰州: 0.5 %兰州: 0.5 %北京: 14.3 %北京: 14.3 %北海: 0.3 %北海: 0.3 %南阳: 0.3 %南阳: 0.3 %台北: 0.3 %台北: 0.3 %台州: 0.3 %台州: 0.3 %吉林: 0.3 %吉林: 0.3 %呼和浩特: 0.3 %呼和浩特: 0.3 %咸宁: 0.3 %咸宁: 0.3 %嘉兴: 0.3 %嘉兴: 0.3 %大庆: 0.3 %大庆: 0.3 %天津: 0.3 %天津: 0.3 %屯昌: 0.3 %屯昌: 0.3 %成都: 0.5 %成都: 0.5 %朝阳: 0.5 %朝阳: 0.5 %杭州: 0.8 %杭州: 0.8 %枣庄: 0.3 %枣庄: 0.3 %楚雄: 0.3 %楚雄: 0.3 %武汉: 0.3 %武汉: 0.3 %洛阳: 0.3 %洛阳: 0.3 %济南: 0.3 %济南: 0.3 %海口: 0.3 %海口: 0.3 %漯河: 0.3 %漯河: 0.3 %秦皇岛: 0.3 %秦皇岛: 0.3 %肇庆: 0.3 %肇庆: 0.3 %芒廷维尤: 20.6 %芒廷维尤: 20.6 %衢州: 0.3 %衢州: 0.3 %西宁: 33.4 %西宁: 33.4 %西安: 0.3 %西安: 0.3 %贵港: 0.3 %贵港: 0.3 %连云港: 0.3 %连云港: 0.3 %重庆: 0.3 %重庆: 0.3 %银川: 0.5 %银川: 0.5 %长沙: 1.0 %长沙: 1.0 %青岛: 0.5 %青岛: 0.5 %香港特别行政区: 0.5 %香港特别行政区: 0.5 %其他ChinaIndianapolisKorea Republic ofTaiwan, China[]上海东莞东营兰州北京北海南阳台北台州吉林呼和浩特咸宁嘉兴大庆天津屯昌成都朝阳杭州枣庄楚雄武汉洛阳济南海口漯河秦皇岛肇庆芒廷维尤衢州西宁西安贵港连云港重庆银川长沙青岛香港特别行政区Highcharts.com
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(3117) PDF downloads(169) Cited by()

Related
Proportional views

Determination of vitaminB6,phenobarbital,chlorphenamine maleate and diazepam in compound benxiaonamin tablets by HPLC

Abstract: Objective: To develop an HPLC quantitative method for the determination of vitaminB6,phenobarbital,chlorphena- mine maleate and diazepam in compound benxiaonamin tablets. Methods: The chromatographic conditions include column C18 (150 mm×4.6 mm,4.6μm);mobile phase A:aeetonitrile-water-triethylamine(100:900:1,contain 0.01 mol/L Sodium Heptane, adjusted to pH 3.5 by acetic aeid);mobile phase B:aeetonitrile,gradient elution:0~12 min,A:100%~70%,12~20 min,A: 70%.The flow rate was 1 mL/min and monitored at 260 nm. Results: The retention time of vitaminB6,phenobarbilal,chlorphena- mine maleate and diazepam were 4.6 min,10.2 min,12.3 min and 14.3 min respectively.The regress equation for VitaminB6 was y=251.4x+2.403,r=0.999 8,and the linear range was0.1684~2.021μg,phenobarbital y=145.8x+1.612,r=0.9999 and the linear range was 0.510 8~6.130μg;ehlorphenamine maleate y=697.3x-7.252,r=0.999 9,and the linear range was 0.122 8~1.474μg,diazepam y=2767x+17.75,r=0.999 8 and the linear range was 0.049 60~0.595 2μg.The average re- covery of vitaminB6,phenobarbital,chlorphenamine maleate and diazepam were 100.1%,99.4%,99.3% and 100.7%;and RSD were 0.18%,0.13%,0.47% and 0.16% respectively. Conclusion:: The method was sensiteve,time-saving and accurate.

YAN Fei, ZHANG Qiao-yan, ZHANG Hong, HAN Ting, JIAO Lei, QIN Lu-ping. Synergistic protective effect of Fructus Schisandrae chinensis with Astragalus polysaccharides on paracetamol-induced acute liver injury in mice[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(5): 340-344.
Citation: TAN Sheng-jian, LIU Gang, WANG Hai-tao, ZHAO Miao, JIANG ren, ZHANG Hua. Determination of vitaminB6,phenobarbital,chlorphenamine maleate and diazepam in compound benxiaonamin tablets by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (4): 286-289.

Catalog

/

DownLoad:  Full-Size Img  PowerPoint
Return
Return