Determination of vitaminB6,phenobarbital,chlorphenamine maleate and diazepam in compound benxiaonamin tablets by HPLC
-
Key words:
- HPLC /
- compound benxiaonamin tablels /
- vitaminB6 /
- phenobarbital /
- chlorphenamine maleate /
- diazepa
Abstract: Objective: To develop an HPLC quantitative method for the determination of vitaminB6,phenobarbital,chlorphena- mine maleate and diazepam in compound benxiaonamin tablets. Methods: The chromatographic conditions include column C18 (150 mm×4.6 mm,4.6μm);mobile phase A:aeetonitrile-water-triethylamine(100:900:1,contain 0.01 mol/L Sodium Heptane, adjusted to pH 3.5 by acetic aeid);mobile phase B:aeetonitrile,gradient elution:0~12 min,A:100%~70%,12~20 min,A: 70%.The flow rate was 1 mL/min and monitored at 260 nm. Results: The retention time of vitaminB6,phenobarbilal,chlorphena- mine maleate and diazepam were 4.6 min,10.2 min,12.3 min and 14.3 min respectively.The regress equation for VitaminB6 was y=251.4x+2.403,r=0.999 8,and the linear range was0.1684~2.021μg,phenobarbital y=145.8x+1.612,r=0.9999 and the linear range was 0.510 8~6.130μg;ehlorphenamine maleate y=697.3x-7.252,r=0.999 9,and the linear range was 0.122 8~1.474μg,diazepam y=2767x+17.75,r=0.999 8 and the linear range was 0.049 60~0.595 2μg.The average re- covery of vitaminB6,phenobarbital,chlorphenamine maleate and diazepam were 100.1%,99.4%,99.3% and 100.7%;and RSD were 0.18%,0.13%,0.47% and 0.16% respectively. Conclusion:: The method was sensiteve,time-saving and accurate.
Citation: | TAN Sheng-jian, LIU Gang, WANG Hai-tao, ZHAO Miao, JIANG ren, ZHANG Hua. Determination of vitaminB6,phenobarbital,chlorphenamine maleate and diazepam in compound benxiaonamin tablets by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (4): 286-289. |