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SUN Yan, ZHOU Xiao-hing, XIE Mu-mu. Determine of the Fengdima eye drops by RP-HPLCC[J]. Journal of Pharmaceutical Practice and Service, 2008, (4): 294-296.
Citation: SUN Yan, ZHOU Xiao-hing, XIE Mu-mu. Determine of the Fengdima eye drops by RP-HPLCC[J]. Journal of Pharmaceutical Practice and Service, 2008, (4): 294-296.

Determine of the Fengdima eye drops by RP-HPLCC

  • Received Date: 2008-07-08
  • Objective: To establish a RP-HPLC method for determining the contents of tetracaine hydrochloride and ephedrine hydrochloride in Fengdima eye drops. Methods: A gradient elution RP-HPLC method was used with a C18column(4.6 mm×250 mm, 5μm),the mobile phase consisted of 0.05 mol/l,sodium dihydrogen phosphate-acetonitril (9:1,for A pump) and 0.05 mol/L sodi- um dihydrogen phosphate buffer-acetonitril (3:7,for B pump);the determination wavelength was 218 nm. Results: The determination was not interfered by the exeipient.The linear range of tetracaine hydrochloride was 50.26~753.9μg/mL,r=1.000.The linear range of ephedrine hydrochloride was 51.38~770.7μg/mL,r=0.999 5.The average recovery were 100.25%,99.57% respectively, while RSD were 0.75%,0.91%.respectively. Conclusion:: The contents of tetracaine hydrochloride and ephedrine hydrochloride could be determined effectively at the same time by the proposed method,which is simple,accurate and can he applied for determining the contents of preparation interfered by honey.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Determine of the Fengdima eye drops by RP-HPLCC

Abstract: Objective: To establish a RP-HPLC method for determining the contents of tetracaine hydrochloride and ephedrine hydrochloride in Fengdima eye drops. Methods: A gradient elution RP-HPLC method was used with a C18column(4.6 mm×250 mm, 5μm),the mobile phase consisted of 0.05 mol/l,sodium dihydrogen phosphate-acetonitril (9:1,for A pump) and 0.05 mol/L sodi- um dihydrogen phosphate buffer-acetonitril (3:7,for B pump);the determination wavelength was 218 nm. Results: The determination was not interfered by the exeipient.The linear range of tetracaine hydrochloride was 50.26~753.9μg/mL,r=1.000.The linear range of ephedrine hydrochloride was 51.38~770.7μg/mL,r=0.999 5.The average recovery were 100.25%,99.57% respectively, while RSD were 0.75%,0.91%.respectively. Conclusion:: The contents of tetracaine hydrochloride and ephedrine hydrochloride could be determined effectively at the same time by the proposed method,which is simple,accurate and can he applied for determining the contents of preparation interfered by honey.

SUN Yan, ZHOU Xiao-hing, XIE Mu-mu. Determine of the Fengdima eye drops by RP-HPLCC[J]. Journal of Pharmaceutical Practice and Service, 2008, (4): 294-296.
Citation: SUN Yan, ZHOU Xiao-hing, XIE Mu-mu. Determine of the Fengdima eye drops by RP-HPLCC[J]. Journal of Pharmaceutical Practice and Service, 2008, (4): 294-296.

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