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FAN Rong, ZHANG Chun, GUAN Hao-yue, PIAO Shu-juan. Content determination of citrate in sodium citrate injection for catheter locking by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (5): 354-356.
Citation:
FAN Rong, ZHANG Chun, GUAN Hao-yue, PIAO Shu-juan. Content determination of citrate in sodium citrate injection for catheter locking by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (5): 354-356.
Content determination of citrate in sodium citrate injection for catheter locking by HPLC
Department of Pharmacy,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China
Received Date: 2007-09-27
Abstract
Objective:To establish a content determination method of citrate in sodium citrate injection for catheter locking. Methods:HPLC was used to determine citrate on Diamonsil C18 column(250 mm×4.6 mm,5μm),using MeOH:0.05 mol/L KH2PO4(adjusted to pH 2.5~2.8 by H3PO4)(5:95,v:v)as a mobile phase with a flow rate at 1.0 mL/min and UV wavelengh of 210 nm.Results:The calibration curve of citrate was line in the range of 0.5~6 mg/mL(r=0.999 9,n=5).The average recovery was 98.79% (RSD=1.08%).The relative contents of citrate in three batch of citrate in sodium citrate injection for catheter locking were 100.51%,98.22% and 97.78%,respectively.Conclusion:This method was simple,rapid,accurate and suitable for the quality control of citrate.
Abstract: Objective:To establish a content determination method of citrate in sodium citrate injection for catheter locking. Methods:HPLC was used to determine citrate on Diamonsil C18 column(250 mm×4.6 mm,5μm),using MeOH:0.05 mol/L KH2PO4(adjusted to pH 2.5~2.8 by H3PO4)(5:95,v:v)as a mobile phase with a flow rate at 1.0 mL/min and UV wavelengh of 210 nm.Results:The calibration curve of citrate was line in the range of 0.5~6 mg/mL(r=0.999 9,n=5).The average recovery was 98.79% (RSD=1.08%).The relative contents of citrate in three batch of citrate in sodium citrate injection for catheter locking were 100.51%,98.22% and 97.78%,respectively.Conclusion:This method was simple,rapid,accurate and suitable for the quality control of citrate.
FAN Rong, ZHANG Chun, GUAN Hao-yue, PIAO Shu-juan. Content determination of citrate in sodium citrate injection for catheter locking by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (5): 354-356.
Citation:
FAN Rong, ZHANG Chun, GUAN Hao-yue, PIAO Shu-juan. Content determination of citrate in sodium citrate injection for catheter locking by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (5): 354-356.
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FAN Rong, ZHANG Chun, GUAN Hao-yue, PIAO Shu-juan. Content determination of citrate in sodium citrate injection for catheter locking by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (5): 354-356.
FAN Rong, ZHANG Chun, GUAN Hao-yue, PIAO Shu-juan. Content determination of citrate in sodium citrate injection for catheter locking by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (5): 354-356.