Determination of baicalin in Xiaoer Runfei Oral liquid by HPLC
- Received Date: 2007-08-03
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Key words:
- HPLC /
- Xiaoer Runfei Oral liquid /
- baicalin
Abstract: Objective: To develop an HPLC quantitative method for the determination of baicalin in Xiaoer Runfei Oral liquid. Methods: HPLC system consisted an Agilent C18column(ZORBAX Eclipse XDB-C18,4.6 mm×150 mm,5μm)and a mixture of methanol-water-phosphoric acid(38:62:0.5)as mobile phase.The flow rate was 1.0 mL/min and was monitored at 278 nm. Results: The retention time of baicalin was 20.6 min.The regress equation for baicalin was Y=0.060 47X+1.131,r=0.999 9,and the linear range was 62.84~314.2μg/mL.The average recovery of baicalin was(n=6)103.3%,RSD 0.43%. Conclusion: The method was sensitive,time-saving and accurate.
Citation: | JIANG Ren, LIU Gang, ZHANG Hua, CHANG Hua, TAN Sheng-jian. Determination of baicalin in Xiaoer Runfei Oral liquid by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2008, (6): 435-437. |