Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code

XU Hong-lei, ZHOU Ting-ting, FAN Guo-rong. Analysis on chemical constituents of hawthorn leaves by HPLC-DAD/ESI-MS[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 40-42.
Citation: XU Hong-lei, ZHOU Ting-ting, FAN Guo-rong. Analysis on chemical constituents of hawthorn leaves by HPLC-DAD/ESI-MS[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 40-42.

Analysis on chemical constituents of hawthorn leaves by HPLC-DAD/ESI-MS

  • Received Date: 2008-04-09
  • Objective : To analyze the chemical constituents of hawthorn leaves by high-performance liquid chromatography(HPLC)-diode array detector/electrospray ionization-mass spectrometry(HPLC-DAD/ESI-MS). Methods : Dried hawthorn leaves were extracted under reflux with 60% ethanol and purified by D101 macroporous resin again.The chromatographic separation was performed on a YMC ODS-C18 column(250 mm×4.6 mm i.d.;5 μm) with a mobile phase composed of aceonitrile-0.1% formic acid(1585 v/v),eluted at a flow rate of 0.6 mL/min,and UV detection was set at 254 nm.The negative ionization mode with needle voltage 3 500 V,capillary voltage-20V,gas(N2)press 21 psi and temperature of the capillary 300 ℃ was selected.Relative molecular mass data acquisition was performed from m/z 300 to 800 in full MS scan mode. Results :Eleven of the major chemical constituents of hawthorn leaves were characterized based on their retention behavior obtained on-line by their UV spectra and the HPLC-DAD/ESI-MS. Conclusion :A rapid and efficient method for studying the chemical constituents of hawthorn leaves by HPLC-DAD/ESI-MS was established.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(2496) PDF downloads(1097) Cited by()

Related
Proportional views

Analysis on chemical constituents of hawthorn leaves by HPLC-DAD/ESI-MS

Abstract: Objective : To analyze the chemical constituents of hawthorn leaves by high-performance liquid chromatography(HPLC)-diode array detector/electrospray ionization-mass spectrometry(HPLC-DAD/ESI-MS). Methods : Dried hawthorn leaves were extracted under reflux with 60% ethanol and purified by D101 macroporous resin again.The chromatographic separation was performed on a YMC ODS-C18 column(250 mm×4.6 mm i.d.;5 μm) with a mobile phase composed of aceonitrile-0.1% formic acid(1585 v/v),eluted at a flow rate of 0.6 mL/min,and UV detection was set at 254 nm.The negative ionization mode with needle voltage 3 500 V,capillary voltage-20V,gas(N2)press 21 psi and temperature of the capillary 300 ℃ was selected.Relative molecular mass data acquisition was performed from m/z 300 to 800 in full MS scan mode. Results :Eleven of the major chemical constituents of hawthorn leaves were characterized based on their retention behavior obtained on-line by their UV spectra and the HPLC-DAD/ESI-MS. Conclusion :A rapid and efficient method for studying the chemical constituents of hawthorn leaves by HPLC-DAD/ESI-MS was established.

XU Hong-lei, ZHOU Ting-ting, FAN Guo-rong. Analysis on chemical constituents of hawthorn leaves by HPLC-DAD/ESI-MS[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 40-42.
Citation: XU Hong-lei, ZHOU Ting-ting, FAN Guo-rong. Analysis on chemical constituents of hawthorn leaves by HPLC-DAD/ESI-MS[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 40-42.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return