Analysis on chemical constituents of hawthorn leaves by HPLC-DAD/ESI-MS
- Received Date: 2008-04-09
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Key words:
- hawthorn leaves /
- HPLC-DAD/ESI-MS /
- chemical constituents
Abstract: Objective : To analyze the chemical constituents of hawthorn leaves by high-performance liquid chromatography(HPLC)-diode array detector/electrospray ionization-mass spectrometry(HPLC-DAD/ESI-MS). Methods : Dried hawthorn leaves were extracted under reflux with 60% ethanol and purified by D101 macroporous resin again.The chromatographic separation was performed on a YMC ODS-C18 column(250 mm×4.6 mm i.d.;5 μm) with a mobile phase composed of aceonitrile-0.1% formic acid(1585 v/v),eluted at a flow rate of 0.6 mL/min,and UV detection was set at 254 nm.The negative ionization mode with needle voltage 3 500 V,capillary voltage-20V,gas(N2)press 21 psi and temperature of the capillary 300 ℃ was selected.Relative molecular mass data acquisition was performed from m/z 300 to 800 in full MS scan mode. Results :Eleven of the major chemical constituents of hawthorn leaves were characterized based on their retention behavior obtained on-line by their UV spectra and the HPLC-DAD/ESI-MS. Conclusion :A rapid and efficient method for studying the chemical constituents of hawthorn leaves by HPLC-DAD/ESI-MS was established.
Citation: | XU Hong-lei, ZHOU Ting-ting, FAN Guo-rong. Analysis on chemical constituents of hawthorn leaves by HPLC-DAD/ESI-MS[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 40-42. |