Studies of isolation for total flavonoids in Rhodobryum giganteum by polyamide column adsorption chromatogtaphy
- Received Date: 2008-06-18
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Key words:
- Rhodobryum giganteum(Schwaegr.) Par. /
- polyamide /
- total flavonoids
Abstract: Objective : To select the optimal condition of polyamide column adsorption chromatogtaphy for separating the total flavonoids in Rhodobryum giganteum(Schwaegr.) Par. Methods :The amount of polyamide,the eluting reagent and the eluting rate were studied. Results :It was suitable when the herb amount:polyamide amount was 11,and 50% ethnoal was the sutiable eluting reagent.The flow rate of eluent was 2 times volume of column/h. Conclusion :polyamide is suitable for the separation and collection of the total flavonoids from Rhodobryum giganteum(Schwaegr.) Par.
Citation: | CAI Ying, LU Xiao-he, WEI Qun-Li. Studies of isolation for total flavonoids in Rhodobryum giganteum by polyamide column adsorption chromatogtaphy[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 58-60. |