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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2009  >  27(1): 63-65

SHEN Dong-yue, PIAO Shu-juan, LIN Hou-wen, TANG Zhen, ZHANG Heng-bi. Determination of flavonoids in Potentilla chinensis ser by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 63-65.
Citation: SHEN Dong-yue, PIAO Shu-juan, LIN Hou-wen, TANG Zhen, ZHANG Heng-bi. Determination of flavonoids in Potentilla chinensis ser by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 63-65.
shu

Determination of flavonoids in Potentilla chinensis ser by HPLC

  • 1.

    Health Sciene Center, Yanbian University, Yanji 133000, China; Department of Pharmacy, ChangzhengHospital, Second Military Medical University, Shanghai 200003, China

  • 2.

    Department of Pharmacy, ChangzhengHospital, Second Military Medical University, Shanghai 200003, China

  • 3.

    208th hospital of PLA, Changchun 130061, China

  • Received Date: 2008-04-01
  • Objective : To develop a HPLC method to determine the content of 3 flavonoids in Potentilla chinensis ser. Methods : ZORBAX SB-C18 column(4.6mm×150mm,5μm) was used,and the mobile phase was methanol-0.3% Phosphoric acid(51:49) with the flow rate of 0.9 mL/min and the detection wanelength at 360 nm. Results :The linear ranges of quercetin,potengriffioside A and apigenin were 0.72～10.08 μg/mL,0.86～12.04μg/mL and 0.54～7.56μg/mL,respectively(r= 0.999 9);the average recovery was 95.37%,96.87% and 97.66%,the RSD was 1.79%,1.63% and 2.08%,respectively;the content of these three flavonoids in the herb was 100μg/g、323μg/g and 55μg/g,respectively. Conclusion :This method is fast,and reliable convenient,which refers to the assay of flavanoids in Potentilla chinensis.
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Determination of flavonoids in Potentilla chinensis ser by HPLC

  • 1. Health Sciene Center, Yanbian University, Yanji 133000, China; Department of Pharmacy, ChangzhengHospital, Second Military Medical University, Shanghai 200003, China
  • 2. Department of Pharmacy, ChangzhengHospital, Second Military Medical University, Shanghai 200003, China
  • 3. 208th hospital of PLA, Changchun 130061, China
  • Received Date: 2008-04-01

Abstract: Objective : To develop a HPLC method to determine the content of 3 flavonoids in Potentilla chinensis ser. Methods : ZORBAX SB-C18 column(4.6mm×150mm,5μm) was used,and the mobile phase was methanol-0.3% Phosphoric acid(51:49) with the flow rate of 0.9 mL/min and the detection wanelength at 360 nm. Results :The linear ranges of quercetin,potengriffioside A and apigenin were 0.72～10.08 μg/mL,0.86～12.04μg/mL and 0.54～7.56μg/mL,respectively(r= 0.999 9);the average recovery was 95.37%,96.87% and 97.66%,the RSD was 1.79%,1.63% and 2.08%,respectively;the content of these three flavonoids in the herb was 100μg/g、323μg/g and 55μg/g,respectively. Conclusion :This method is fast,and reliable convenient,which refers to the assay of flavanoids in Potentilla chinensis.

SHEN Dong-yue, PIAO Shu-juan, LIN Hou-wen, TANG Zhen, ZHANG Heng-bi. Determination of flavonoids in Potentilla chinensis ser by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 63-65.
Citation: SHEN Dong-yue, PIAO Shu-juan, LIN Hou-wen, TANG Zhen, ZHANG Heng-bi. Determination of flavonoids in Potentilla chinensis ser by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 63-65.
shu

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