Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code

SHEN Dong-yue, PIAO Shu-juan, LIN Hou-wen, TANG Zhen, ZHANG Heng-bi. Determination of flavonoids in Potentilla chinensis ser by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 63-65.
Citation: SHEN Dong-yue, PIAO Shu-juan, LIN Hou-wen, TANG Zhen, ZHANG Heng-bi. Determination of flavonoids in Potentilla chinensis ser by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 63-65.

Determination of flavonoids in Potentilla chinensis ser by HPLC

  • Received Date: 2008-04-01
  • Objective : To develop a HPLC method to determine the content of 3 flavonoids in Potentilla chinensis ser. Methods : ZORBAX SB-C18 column(4.6mm×150mm,5μm) was used,and the mobile phase was methanol-0.3% Phosphoric acid(51:49) with the flow rate of 0.9 mL/min and the detection wanelength at 360 nm. Results :The linear ranges of quercetin,potengriffioside A and apigenin were 0.72~10.08 μg/mL,0.86~12.04μg/mL and 0.54~7.56μg/mL,respectively(r= 0.999 9);the average recovery was 95.37%,96.87% and 97.66%,the RSD was 1.79%,1.63% and 2.08%,respectively;the content of these three flavonoids in the herb was 100μg/g、323μg/g and 55μg/g,respectively. Conclusion :This method is fast,and reliable convenient,which refers to the assay of flavanoids in Potentilla chinensis.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(2245) PDF downloads(531) Cited by()

Related
Proportional views

Determination of flavonoids in Potentilla chinensis ser by HPLC

Abstract: Objective : To develop a HPLC method to determine the content of 3 flavonoids in Potentilla chinensis ser. Methods : ZORBAX SB-C18 column(4.6mm×150mm,5μm) was used,and the mobile phase was methanol-0.3% Phosphoric acid(51:49) with the flow rate of 0.9 mL/min and the detection wanelength at 360 nm. Results :The linear ranges of quercetin,potengriffioside A and apigenin were 0.72~10.08 μg/mL,0.86~12.04μg/mL and 0.54~7.56μg/mL,respectively(r= 0.999 9);the average recovery was 95.37%,96.87% and 97.66%,the RSD was 1.79%,1.63% and 2.08%,respectively;the content of these three flavonoids in the herb was 100μg/g、323μg/g and 55μg/g,respectively. Conclusion :This method is fast,and reliable convenient,which refers to the assay of flavanoids in Potentilla chinensis.

SHEN Dong-yue, PIAO Shu-juan, LIN Hou-wen, TANG Zhen, ZHANG Heng-bi. Determination of flavonoids in Potentilla chinensis ser by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 63-65.
Citation: SHEN Dong-yue, PIAO Shu-juan, LIN Hou-wen, TANG Zhen, ZHANG Heng-bi. Determination of flavonoids in Potentilla chinensis ser by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(1): 63-65.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return