Determination of two amino acids in human fibrin sealant kit by pre-column derivatization HPLC
- Received Date: 2008-12-31
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Key words:
- arginine hydrochloride /
- leucine /
- HPLC /
- derivation /
- 2,4-dinitrofluorobenzene
Abstract: Objective :To set up a method for determination of arginine and leucine in human fibrin sealant kit. Methods :A Shim-pack CLC-ODS column was used after pre-column derivatization with 2,4-dinitrobenzene fluoride,with the mobile phase of 50% acetonitrile and 0.05mol/L acetate buffer,and the UV detection wavelength was 362 nm.The content was analyzed by external standard method. Results :These two amino acid derivatives can be well separated under the chromatographic conditions.The average recovery was 102.8%(RSD=0.83%) for Arg,and 103.8%(RSD=0.92%) for Leu. Conclusion :The method is accurate,convenient,low-cost and suitable for determination of the two amino-acids in human fibrin sealant kit.
Citation: | ZHAO Yan-hong, WANG Qiang, XU Li. Determination of two amino acids in human fibrin sealant kit by pre-column derivatization HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(3): 198-200. |