Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code

ZHAO Yan-hong, WANG Qiang, XU Li. Determination of two amino acids in human fibrin sealant kit by pre-column derivatization HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(3): 198-200.
Citation: ZHAO Yan-hong, WANG Qiang, XU Li. Determination of two amino acids in human fibrin sealant kit by pre-column derivatization HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(3): 198-200.

Determination of two amino acids in human fibrin sealant kit by pre-column derivatization HPLC

  • Received Date: 2008-12-31
  • Objective :To set up a method for determination of arginine and leucine in human fibrin sealant kit. Methods :A Shim-pack CLC-ODS column was used after pre-column derivatization with 2,4-dinitrobenzene fluoride,with the mobile phase of 50% acetonitrile and 0.05mol/L acetate buffer,and the UV detection wavelength was 362 nm.The content was analyzed by external standard method. Results :These two amino acid derivatives can be well separated under the chromatographic conditions.The average recovery was 102.8%(RSD=0.83%) for Arg,and 103.8%(RSD=0.92%) for Leu. Conclusion :The method is accurate,convenient,low-cost and suitable for determination of the two amino-acids in human fibrin sealant kit.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(2073) PDF downloads(741) Cited by()

Related
Proportional views

Determination of two amino acids in human fibrin sealant kit by pre-column derivatization HPLC

Abstract: Objective :To set up a method for determination of arginine and leucine in human fibrin sealant kit. Methods :A Shim-pack CLC-ODS column was used after pre-column derivatization with 2,4-dinitrobenzene fluoride,with the mobile phase of 50% acetonitrile and 0.05mol/L acetate buffer,and the UV detection wavelength was 362 nm.The content was analyzed by external standard method. Results :These two amino acid derivatives can be well separated under the chromatographic conditions.The average recovery was 102.8%(RSD=0.83%) for Arg,and 103.8%(RSD=0.92%) for Leu. Conclusion :The method is accurate,convenient,low-cost and suitable for determination of the two amino-acids in human fibrin sealant kit.

ZHAO Yan-hong, WANG Qiang, XU Li. Determination of two amino acids in human fibrin sealant kit by pre-column derivatization HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(3): 198-200.
Citation: ZHAO Yan-hong, WANG Qiang, XU Li. Determination of two amino acids in human fibrin sealant kit by pre-column derivatization HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(3): 198-200.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return