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Volume 27 Issue 3
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2009  >  27(3): 201-204

XU Li-li, ZHAO Liang, XIA Hui, ZHANG Guo-qing. Simultaneous determination of four steroidal saponins compounds in Paris ployphylla by RP-HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(3): 201-204.
Citation: XU Li-li, ZHAO Liang, XIA Hui, ZHANG Guo-qing. Simultaneous determination of four steroidal saponins compounds in Paris ployphylla by RP-HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(3): 201-204.
shu

Simultaneous determination of four steroidal saponins compounds in Paris ployphylla by RP-HPLC method

  • 1.

    Department of Pharmacy, Eastern Hepatobilliary Hospital, Second Military Medical University, Shanghai 200438, China

  • 2.

    School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2008-09-12
  • Objective :To establish a simultaneous determination method of steroidal saponin Ⅰ、Ⅱ、Ⅵ、Ⅶ in Paris ployphylla by RP-HPLC. Methods :The column was Agilent SB C18(4.6 mm×150 mm,5 μm).The mobile phase was acetonitrile-water with gradient elution at a flow rate of 0.8 mL/min.The UV detection was performed at 210 nm. Results :The calibration curves showed good linearity(r>0.999 8,n=5).The recoveries of the four steroidal saponins were from 98.12% to 104.3%.The limits of detection(LOD) ranged from 11.8 to 21.0 ng and limits of quantification(LOQ) ranged from 38.4 to 75.0 ng for all the reference standards,respectively.The results showed that the contents of the four steroidal saponins in Paris polyphylla very from different producing area. Conclusion :The method is accurate,convenient,and suitable for the quantitative analysis of four saponins from Paris ployphylla.
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Simultaneous determination of four steroidal saponins compounds in Paris ployphylla by RP-HPLC method

  • 1. Department of Pharmacy, Eastern Hepatobilliary Hospital, Second Military Medical University, Shanghai 200438, China
  • 2. School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2008-09-12

Abstract: Objective :To establish a simultaneous determination method of steroidal saponin Ⅰ、Ⅱ、Ⅵ、Ⅶ in Paris ployphylla by RP-HPLC. Methods :The column was Agilent SB C18(4.6 mm×150 mm,5 μm).The mobile phase was acetonitrile-water with gradient elution at a flow rate of 0.8 mL/min.The UV detection was performed at 210 nm. Results :The calibration curves showed good linearity(r>0.999 8,n=5).The recoveries of the four steroidal saponins were from 98.12% to 104.3%.The limits of detection(LOD) ranged from 11.8 to 21.0 ng and limits of quantification(LOQ) ranged from 38.4 to 75.0 ng for all the reference standards,respectively.The results showed that the contents of the four steroidal saponins in Paris polyphylla very from different producing area. Conclusion :The method is accurate,convenient,and suitable for the quantitative analysis of four saponins from Paris ployphylla.

XU Li-li, ZHAO Liang, XIA Hui, ZHANG Guo-qing. Simultaneous determination of four steroidal saponins compounds in Paris ployphylla by RP-HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(3): 201-204.
Citation: XU Li-li, ZHAO Liang, XIA Hui, ZHANG Guo-qing. Simultaneous determination of four steroidal saponins compounds in Paris ployphylla by RP-HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(3): 201-204.
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