Simultaneous determination of five flavone constituents in Epimedium Brevicornum by HPLC

XIA Ai-jun; HUANG He-zhou; GAO Gang; ZHANG Hai; ZHU Zhen-yu; CHAI Yi-feng

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Volume 27 Issue 4

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Article Navigation > Journal of Pharmaceutical Practice and Service > 2009 > 27(4): 284-285,288

XIA Ai-jun, HUANG He-zhou, GAO Gang, ZHANG Hai, ZHU Zhen-yu, CHAI Yi-feng. Simultaneous determination of five flavone constituents in Epimedium Brevicornum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 284-285,288.

Citation:

XIA Ai-jun, HUANG He-zhou, GAO Gang, ZHANG Hai, ZHU Zhen-yu, CHAI Yi-feng. Simultaneous determination of five flavone constituents in Epimedium Brevicornum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 284-285,288.

Simultaneous determination of five flavone constituents in Epimedium Brevicornum by HPLC

1.
Department of pharmacy, 303th Hospital of PLA, Nanning 530021, China
2.
School of pharmacy, Second Military Medical University, Shanghai 200433, China
3.
Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China

Received Date: 2008-11-20

Abstract

Objective : To establish a HPLC method for simultaneous determination of five flavone constituents in Epimedium Brevicornum. Methods :The determination was performed on Diamonsil-C₁₈ column(4.6mm×250mm,5μm,Dikma).The mobile phase was consisted of acetonitrile-formic acid aqueous solution with a flow rate of 0.9 mL/min.The detection wavelength was set at 270 nm. Results :A method has been established for determination of epimedin A₁,epimedin B,icariin,sagittatoside A,baohuoside I in one run by HPLC. Conclusion :The established method is simple,convenient,accurate and reliable,and suitable for the quality control of Epimedium Brevicornum.
- epimedin A1,
- epimedin B,
- icariin,
- sagittatoside A,
- baohuoside I,
- HPLC
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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