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LIU Li, LONG Ping, YAO Jian-zhong. Determination of luteolin in three kinds of Chrysanthemum morifolium by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 289-290,293.
Citation: LIU Li, LONG Ping, YAO Jian-zhong. Determination of luteolin in three kinds of Chrysanthemum morifolium by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 289-290,293.

Determination of luteolin in three kinds of Chrysanthemum morifolium by HPLC

  • Received Date: 2009-01-06
  • Objective :To determine the content of luteolin in three kinds of Chrysanthemum morifolium that are often seen in market by HPLC. Methods : The luteolin was quantified by reverse-HPLC on Diamosil C18 column(250 mm×4.6 mm,5 μm)using methanol-water(changed from 50∶50 to 80∶20 in 30 min at pace) as mobile phase with a flow rate of 1 mL/min.The detecting wavelength was 350 nm. Results :The linear range of luteolin was 0.224 ~2.24 μg(r=0.999 9).The average recovery was 99.40%,and RSD was 2.67%(n=9).The average content of luteolin in Hangju,Gongju and Haoju was 0.34,0.41,0.69 mg/g,and RSD was 0.8%,1.2%,1.3%,respectively.(n=6). Conclusion :The content of luteolin in Haoju was the highest.The established method is delicate,simple,convenient,accurate,reproducible,and suitable for the quality control of luteolin in defferent kinds of Chrysanthemum morifolium that are often seen in market.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Determination of luteolin in three kinds of Chrysanthemum morifolium by HPLC

Abstract: Objective :To determine the content of luteolin in three kinds of Chrysanthemum morifolium that are often seen in market by HPLC. Methods : The luteolin was quantified by reverse-HPLC on Diamosil C18 column(250 mm×4.6 mm,5 μm)using methanol-water(changed from 50∶50 to 80∶20 in 30 min at pace) as mobile phase with a flow rate of 1 mL/min.The detecting wavelength was 350 nm. Results :The linear range of luteolin was 0.224 ~2.24 μg(r=0.999 9).The average recovery was 99.40%,and RSD was 2.67%(n=9).The average content of luteolin in Hangju,Gongju and Haoju was 0.34,0.41,0.69 mg/g,and RSD was 0.8%,1.2%,1.3%,respectively.(n=6). Conclusion :The content of luteolin in Haoju was the highest.The established method is delicate,simple,convenient,accurate,reproducible,and suitable for the quality control of luteolin in defferent kinds of Chrysanthemum morifolium that are often seen in market.

LIU Li, LONG Ping, YAO Jian-zhong. Determination of luteolin in three kinds of Chrysanthemum morifolium by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 289-290,293.
Citation: LIU Li, LONG Ping, YAO Jian-zhong. Determination of luteolin in three kinds of Chrysanthemum morifolium by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 289-290,293.

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