Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code

HE Hou-luo, HUANG Zhang-bei, SHENG Jia-yu, YUE Xiao-qiang, LI Min, XIN Hai-liang. Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 291-293.
Citation: HE Hou-luo, HUANG Zhang-bei, SHENG Jia-yu, YUE Xiao-qiang, LI Min, XIN Hai-liang. Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 291-293.

Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng

  • Received Date: 2008-11-05
  • Objective : To determine quantitatively ginsenoside Re in total ginsenoside from stalk and leaf of Panax ginseng,and to provide experimental evident for total ginsenoside quality control of Panax ginseng. Methods :The method was developed with the condition as follows:Diamonsil C18(4.6 mm×25 mm,5 μm) and DIKMA EasyGuard C18(10 mm×4.6 mm) was used as chromatographic column and guard column respectively.Gradient elution was employed with the mobile phase of acetonitrile-0.5% acetic acid/water at a flow rate of 1.25 mL/min.ELSD:temperature of the drift tube 40 ℃;gas pressure: 3.4 bar;gain: 7. Results :The standard curve: C=1.191×10-7A-0.187 6;the linear range: 0.038~1.14 mg/mL,r=0.999 3;the limits of detection: 20 ng(S/N>3);recovery rate of the added sample: 96.84%~104.21%. Conclusion :With its accuracy,sensitivity,reproducibility as well as the simplicity of sample preparation,this method is feasible for quality control of total ginsenoside from stalk and leaf of Panax ginseng.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(3702) PDF downloads(967) Cited by()

Related
Proportional views

Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng

Abstract: Objective : To determine quantitatively ginsenoside Re in total ginsenoside from stalk and leaf of Panax ginseng,and to provide experimental evident for total ginsenoside quality control of Panax ginseng. Methods :The method was developed with the condition as follows:Diamonsil C18(4.6 mm×25 mm,5 μm) and DIKMA EasyGuard C18(10 mm×4.6 mm) was used as chromatographic column and guard column respectively.Gradient elution was employed with the mobile phase of acetonitrile-0.5% acetic acid/water at a flow rate of 1.25 mL/min.ELSD:temperature of the drift tube 40 ℃;gas pressure: 3.4 bar;gain: 7. Results :The standard curve: C=1.191×10-7A-0.187 6;the linear range: 0.038~1.14 mg/mL,r=0.999 3;the limits of detection: 20 ng(S/N>3);recovery rate of the added sample: 96.84%~104.21%. Conclusion :With its accuracy,sensitivity,reproducibility as well as the simplicity of sample preparation,this method is feasible for quality control of total ginsenoside from stalk and leaf of Panax ginseng.

HE Hou-luo, HUANG Zhang-bei, SHENG Jia-yu, YUE Xiao-qiang, LI Min, XIN Hai-liang. Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 291-293.
Citation: HE Hou-luo, HUANG Zhang-bei, SHENG Jia-yu, YUE Xiao-qiang, LI Min, XIN Hai-liang. Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 291-293.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return