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Volume 27 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2009  >  27(4): 291-293

HE Hou-luo, HUANG Zhang-bei, SHENG Jia-yu, YUE Xiao-qiang, LI Min, XIN Hai-liang. Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 291-293.
Citation: HE Hou-luo, HUANG Zhang-bei, SHENG Jia-yu, YUE Xiao-qiang, LI Min, XIN Hai-liang. Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 291-293.
shu

Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng

  • 1.

    Department of Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China

  • 2.

    Department of Military Health, Naval Faculty, Second Military Medical University, Shanghai 200433, China

  • 3.

    Department of Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China; 546th Hospital of PLA, Urumqi 841700, China

  • Received Date: 2008-11-05
  • Objective : To determine quantitatively ginsenoside Re in total ginsenoside from stalk and leaf of Panax ginseng,and to provide experimental evident for total ginsenoside quality control of Panax ginseng. Methods :The method was developed with the condition as follows:Diamonsil C18(4.6 mm×25 mm,5 μm) and DIKMA EasyGuard C18(10 mm×4.6 mm) was used as chromatographic column and guard column respectively.Gradient elution was employed with the mobile phase of acetonitrile-0.5% acetic acid/water at a flow rate of 1.25 mL/min.ELSD:temperature of the drift tube 40 ℃;gas pressure: 3.4 bar;gain: 7. Results :The standard curve: C=1.191×10-7A-0.187 6;the linear range: 0.038～1.14 mg/mL,r=0.999 3;the limits of detection: 20 ng(S/N>3);recovery rate of the added sample: 96.84%～104.21%. Conclusion :With its accuracy,sensitivity,reproducibility as well as the simplicity of sample preparation,this method is feasible for quality control of total ginsenoside from stalk and leaf of Panax ginseng.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng

  • 1. Department of Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China
  • 2. Department of Military Health, Naval Faculty, Second Military Medical University, Shanghai 200433, China
  • 3. Department of Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China; 546th Hospital of PLA, Urumqi 841700, China
  • Received Date: 2008-11-05

Abstract: Objective : To determine quantitatively ginsenoside Re in total ginsenoside from stalk and leaf of Panax ginseng,and to provide experimental evident for total ginsenoside quality control of Panax ginseng. Methods :The method was developed with the condition as follows:Diamonsil C18(4.6 mm×25 mm,5 μm) and DIKMA EasyGuard C18(10 mm×4.6 mm) was used as chromatographic column and guard column respectively.Gradient elution was employed with the mobile phase of acetonitrile-0.5% acetic acid/water at a flow rate of 1.25 mL/min.ELSD:temperature of the drift tube 40 ℃;gas pressure: 3.4 bar;gain: 7. Results :The standard curve: C=1.191×10-7A-0.187 6;the linear range: 0.038～1.14 mg/mL,r=0.999 3;the limits of detection: 20 ng(S/N>3);recovery rate of the added sample: 96.84%～104.21%. Conclusion :With its accuracy,sensitivity,reproducibility as well as the simplicity of sample preparation,this method is feasible for quality control of total ginsenoside from stalk and leaf of Panax ginseng.

HE Hou-luo, HUANG Zhang-bei, SHENG Jia-yu, YUE Xiao-qiang, LI Min, XIN Hai-liang. Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 291-293.
Citation: HE Hou-luo, HUANG Zhang-bei, SHENG Jia-yu, YUE Xiao-qiang, LI Min, XIN Hai-liang. Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 291-293.
shu

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