Inhibition research of three commonly-used anti-tumor drugs against HepG2 cell line
- Received Date: 2009-02-25
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Key words:
- MTT /
- HepG2 /
- IC50 /
- 5-Fu /
- Vincristine /
- Mitomycin C
Abstract: Objective :To establish an inhibition research method of three commonly-used anti-tumor drugs against HepG2 cell line. Methods : The determination was performed on a 96-well plate.The drugs were administrated in a series of concentrations,and each concentration was triplicate.Cells without drugs were set as a negative control.Followed by determination of MTT method,the inhibition ratio was plotted to concentration. Results :The inhibition ability of 3 drugs were different when the concentration were in different range.On the whole,the inhibition ability of 5-Fu was the weakest one. Conclusion :The method evaluates and compares the inhibition ability of 3 commonly-used anti-tumor drugs against HepG2 cell line,and could be a reference in positive control of drug screening experiment.
Citation: | JIA Jing, LI Yue-yue, ZHU Zhen-yu, CHAI Yi-feng, ZHANG Guo-qing. Inhibition research of three commonly-used anti-tumor drugs against HepG2 cell line[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(5): 323-333,361. |