Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 28 Issue 1
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2010  >  28(1): 25-28,69

YE Qi, WANG Jing, GUO Xue-qing, CHEN Chong-hong, ZHANG Jun-ping. Etoposide induces apoptosis via ubiquitin-proteasome pathway in HL-60 leukemia cells[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(1): 25-28,69.
Citation: YE Qi, WANG Jing, GUO Xue-qing, CHEN Chong-hong, ZHANG Jun-ping. Etoposide induces apoptosis via ubiquitin-proteasome pathway in HL-60 leukemia cells[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(1): 25-28,69.
shu

Etoposide induces apoptosis via ubiquitin-proteasome pathway in HL-60 leukemia cells

  • 1.

    Department of Pharmacology,Fujian Medical University,Fuzhou 350004,China;Department of Biomedical Pharmacy,School of Pharmacy,Second Military Medical University,Shanghai 200433,China

  • 2.

    Department of Biomedical Pharmacy,School of Pharmacy,Second Military Medical University,Shanghai 200433,China

  • 3.

    United Gene Holdings,Ltd.,Shanghai,200092,China

  • 4.

    Department of Pharmacology,Fujian Medical University,Fuzhou 350004,China

  • 5.

    Department of Biomedical Pharmacy,School of Pharmacy,Second Military Medical University,Shanghai 200433,China

  • Received Date: 2009-09-17
  • Rev Recd Date: 2009-10-20
  • Objective To investigate the molecular mechanisms of Topoisomerase II inhibitor etopside(induced apoptosis on human leukemia HL-60 cells. Methods Cell growth and apoptosis were determined by MTT assay and flow cytometry.cDNA microarray was used to assess sensitive early gene expression profiles and Genmapp software was used to gene cluster and pathway analysis of the drug response. Results Etopside exhibited cell growth inhibition by 50 % at the concentrations of(30.17±0.26)mol/L and induced cell apoptosis from 4.38% to 53.96%.Genmapp analysis showed that etopside significantly inhibited ubiquitin-proteasome pathway(Z≥3.8) and decreased expression of genes involved in ubiquitin-proteasome pathway(PSMB5、PSMB7、PSMB8、PSMC3、PSMC5、RPN1and HLA(A). Conclusion Etoposide induces apoptosis via decreased expression of genes in ubiquitin-proteasome pathway.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(2720) PDF downloads(803) Cited by()

Related
Proportional views

Etoposide induces apoptosis via ubiquitin-proteasome pathway in HL-60 leukemia cells

  • 1. Department of Pharmacology,Fujian Medical University,Fuzhou 350004,China;Department of Biomedical Pharmacy,School of Pharmacy,Second Military Medical University,Shanghai 200433,China
  • 2. Department of Biomedical Pharmacy,School of Pharmacy,Second Military Medical University,Shanghai 200433,China
  • 3. United Gene Holdings,Ltd.,Shanghai,200092,China
  • 4. Department of Pharmacology,Fujian Medical University,Fuzhou 350004,China
  • 5. Department of Biomedical Pharmacy,School of Pharmacy,Second Military Medical University,Shanghai 200433,China
  • Received Date: 2009-09-17
  • Rev Recd Date: 2009-10-20

Abstract: Objective To investigate the molecular mechanisms of Topoisomerase II inhibitor etopside(induced apoptosis on human leukemia HL-60 cells. Methods Cell growth and apoptosis were determined by MTT assay and flow cytometry.cDNA microarray was used to assess sensitive early gene expression profiles and Genmapp software was used to gene cluster and pathway analysis of the drug response. Results Etopside exhibited cell growth inhibition by 50 % at the concentrations of(30.17±0.26)mol/L and induced cell apoptosis from 4.38% to 53.96%.Genmapp analysis showed that etopside significantly inhibited ubiquitin-proteasome pathway(Z≥3.8) and decreased expression of genes involved in ubiquitin-proteasome pathway(PSMB5、PSMB7、PSMB8、PSMC3、PSMC5、RPN1and HLA(A). Conclusion Etoposide induces apoptosis via decreased expression of genes in ubiquitin-proteasome pathway.

YE Qi, WANG Jing, GUO Xue-qing, CHEN Chong-hong, ZHANG Jun-ping. Etoposide induces apoptosis via ubiquitin-proteasome pathway in HL-60 leukemia cells[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(1): 25-28,69.
Citation: YE Qi, WANG Jing, GUO Xue-qing, CHEN Chong-hong, ZHANG Jun-ping. Etoposide induces apoptosis via ubiquitin-proteasome pathway in HL-60 leukemia cells[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(1): 25-28,69.
shu

    HTML

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return