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Volume 28 Issue 2
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2010  >  28(2): 114-115,153

GONG Yan-ling, HUANG Shan, YU Hui-rong. Enzymatic extraction and antibacterial activity of total flavonoids from Sedum sarmentosum[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(2): 114-115,153.
Citation: GONG Yan-ling, HUANG Shan, YU Hui-rong. Enzymatic extraction and antibacterial activity of total flavonoids from Sedum sarmentosum[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(2): 114-115,153.
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Enzymatic extraction and antibacterial activity of total flavonoids from Sedum sarmentosum

  • Pharmaceutical Department,College of Chemical Engineering,Qingdao University of Science and Technology,Qingdao 266042,China

  • Received Date: 2009-06-29
  • Rev Recd Date: 2009-09-11
  • Objective To investigate the technology of total flavonoids in Sedum sarmentosum extracted by enzymatic method and its antibacterial activity. Methods Total flavonoids in Sedum sarmentosum was extracted by the method that enzymatic hydrolysis combined with water decocting.The extraction technology was optimized by orthogonal design with the content of total flavonoids yield.The antibacterial activity of total flavonoids was determined by stiletto method.The Results reveled that the optimum technology was to be hydrolyzed for 1.5 h by 0.6% enzyme at 50 ℃ in pH 6.0.The significant factor was the temperature of enzymatic hydrolysis.The yield of total flavonoids was 3.471%.Total flavonoids of Sedum sarmentosum was bacteriostasis on E.coli,Staphylococcus aureus,Bacillus subtilis and Bacillus gasoformans.The bacteriostasis on Bacillus aerogenes was not obvious.The extraction technology obtained in this experiment are reasonable and feasible with high extraction rate,and the total flavonoids has some antibacterial activity.
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Enzymatic extraction and antibacterial activity of total flavonoids from Sedum sarmentosum

  • Pharmaceutical Department,College of Chemical Engineering,Qingdao University of Science and Technology,Qingdao 266042,China
  • Received Date: 2009-06-29
  • Rev Recd Date: 2009-09-11

Abstract: Objective To investigate the technology of total flavonoids in Sedum sarmentosum extracted by enzymatic method and its antibacterial activity. Methods Total flavonoids in Sedum sarmentosum was extracted by the method that enzymatic hydrolysis combined with water decocting.The extraction technology was optimized by orthogonal design with the content of total flavonoids yield.The antibacterial activity of total flavonoids was determined by stiletto method.The Results reveled that the optimum technology was to be hydrolyzed for 1.5 h by 0.6% enzyme at 50 ℃ in pH 6.0.The significant factor was the temperature of enzymatic hydrolysis.The yield of total flavonoids was 3.471%.Total flavonoids of Sedum sarmentosum was bacteriostasis on E.coli,Staphylococcus aureus,Bacillus subtilis and Bacillus gasoformans.The bacteriostasis on Bacillus aerogenes was not obvious.The extraction technology obtained in this experiment are reasonable and feasible with high extraction rate,and the total flavonoids has some antibacterial activity.

GONG Yan-ling, HUANG Shan, YU Hui-rong. Enzymatic extraction and antibacterial activity of total flavonoids from Sedum sarmentosum[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(2): 114-115,153.
Citation: GONG Yan-ling, HUANG Shan, YU Hui-rong. Enzymatic extraction and antibacterial activity of total flavonoids from Sedum sarmentosum[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(2): 114-115,153.
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