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YAN Dong-lin. Determination of adenosine in capsule of Chinese Caterpillar Fungus by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(4): 293-295.
Citation: YAN Dong-lin. Determination of adenosine in capsule of Chinese Caterpillar Fungus by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(4): 293-295.

Determination of adenosine in capsule of Chinese Caterpillar Fungus by HPLC

  • Received Date: 2009-11-30
  • Rev Recd Date: 2010-03-18
  • Objective To establish a method for determination of adenosine in capsule of Chinese Caterpillar Fungus. Methods HPLC was carried out with Agilent TC-C18(4.6 mm × 250 mm,5 μm);the Mobile phase was acetonitrile-0.01 mol/L potassium dihydrogen phosphate solution(892);the flow rate was 1.0 ml/min;the detection wavelength was 254 nm;the column temperature was 30 ℃;and content calculation was used external standard method. Results The concentration of adenosine in 0.402 4~60.36 μg/ml(r=1) had good linear relationship with peak area;the average recovery was 98.04%(n=6) with RSD of 0.63%. Conclusion This method is simple,reliable and can be used to quality control of adenosine in capsule of Chinese Caterpillar Fungus.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Determination of adenosine in capsule of Chinese Caterpillar Fungus by HPLC

Abstract: Objective To establish a method for determination of adenosine in capsule of Chinese Caterpillar Fungus. Methods HPLC was carried out with Agilent TC-C18(4.6 mm × 250 mm,5 μm);the Mobile phase was acetonitrile-0.01 mol/L potassium dihydrogen phosphate solution(892);the flow rate was 1.0 ml/min;the detection wavelength was 254 nm;the column temperature was 30 ℃;and content calculation was used external standard method. Results The concentration of adenosine in 0.402 4~60.36 μg/ml(r=1) had good linear relationship with peak area;the average recovery was 98.04%(n=6) with RSD of 0.63%. Conclusion This method is simple,reliable and can be used to quality control of adenosine in capsule of Chinese Caterpillar Fungus.

YAN Dong-lin. Determination of adenosine in capsule of Chinese Caterpillar Fungus by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(4): 293-295.
Citation: YAN Dong-lin. Determination of adenosine in capsule of Chinese Caterpillar Fungus by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(4): 293-295.

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