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HUO Yan, SHI Hai-ming, LI Wei-wei, GUO Cheng, LI Xiao-bo. Determination and structural elucidation of sesquiterpene lactones from Inula racemosa[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(5): 334-338.
Citation: HUO Yan, SHI Hai-ming, LI Wei-wei, GUO Cheng, LI Xiao-bo. Determination and structural elucidation of sesquiterpene lactones from Inula racemosa[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(5): 334-338.

Determination and structural elucidation of sesquiterpene lactones from Inula racemosa

  • Received Date: 2010-03-12
  • Rev Recd Date: 2010-04-07
  • Objective To establish a method of HPLC for the simultaneous quantification of three sesquiterpene lactones in Inula racemosa. Methods Agilent Zorbax XDB-C18 column(250 mm×4.6 mm,5 μm) was uesed with a mobile phase consists of 55% acetonitrile and 45% water,at a flow rate of 1.0 ml/min,and detection wavelength of 210 nm. Results Three sesquiterpene lactones in Inula racemosa Hook.f.,namely igalane(1),isoalantolactone(2) and alantolactone(3) has good linear relation in 17.40~1 740.00,21.20~2 120.00,20.40~2 040.00 μg/ml with the lowest detection limit of 0.087,0.042,0.041 μg/ml respectively.The RSD of repeatability and stability were all less than 2.85%.The average recovery rate of there constituents were all less than 104.43% with RSD less than<1.20%. Conclusion The method was rapid,accurate,reliable and could be suitable as a quality control method for I.racemosa.Two minor isomers from I.racemosa were isolated by semi-preparative HPLC which structures were elucidated on the basis of NMR analysis.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Determination and structural elucidation of sesquiterpene lactones from Inula racemosa

Abstract: Objective To establish a method of HPLC for the simultaneous quantification of three sesquiterpene lactones in Inula racemosa. Methods Agilent Zorbax XDB-C18 column(250 mm×4.6 mm,5 μm) was uesed with a mobile phase consists of 55% acetonitrile and 45% water,at a flow rate of 1.0 ml/min,and detection wavelength of 210 nm. Results Three sesquiterpene lactones in Inula racemosa Hook.f.,namely igalane(1),isoalantolactone(2) and alantolactone(3) has good linear relation in 17.40~1 740.00,21.20~2 120.00,20.40~2 040.00 μg/ml with the lowest detection limit of 0.087,0.042,0.041 μg/ml respectively.The RSD of repeatability and stability were all less than 2.85%.The average recovery rate of there constituents were all less than 104.43% with RSD less than<1.20%. Conclusion The method was rapid,accurate,reliable and could be suitable as a quality control method for I.racemosa.Two minor isomers from I.racemosa were isolated by semi-preparative HPLC which structures were elucidated on the basis of NMR analysis.

HUO Yan, SHI Hai-ming, LI Wei-wei, GUO Cheng, LI Xiao-bo. Determination and structural elucidation of sesquiterpene lactones from Inula racemosa[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(5): 334-338.
Citation: HUO Yan, SHI Hai-ming, LI Wei-wei, GUO Cheng, LI Xiao-bo. Determination and structural elucidation of sesquiterpene lactones from Inula racemosa[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(5): 334-338.

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