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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2011  >  29(1): 15-16,70

Jiang Nan, Yang Yong-Ge, Xu Xue-Ting, Zhao Gang-Tao. Determination of Atorvastatin in human plasma by LC-MS-MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 15-16,70.
Citation: Jiang Nan, Yang Yong-Ge, Xu Xue-Ting, Zhao Gang-Tao. Determination of Atorvastatin in human plasma by LC-MS-MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 15-16,70.
shu

Determination of Atorvastatin in human plasma by LC-MS-MS

  • Department of Pharmacy,General Hospital of Beijing Military Command of PLA,Beijing 100700,China

  • Received Date: 2010-05-18
  • Rev Recd Date: 2010-09-09
  • Objective To establish a method for determining Atorvastatin in human plasma by LC-MS-MS. Methods Atorvastatin was extracted for determination by LC-MS-MS. Analytical column was Thermo BioBasic-C8(2.1 mm×100 mm,5 μm). The mobile phase was acetonitrile(0.1%acid):water(0.1%acid)=70:30. Mass spectrum conditions was ESI performing in the SRM mode using target ions m/z 558→278(30EV)(Atorvastatin ), m/z 269→106(22 EV)(tolbutamide), SP 3 500 KV, SGP 10 Arb, AGP 15 Arb, TEM 314 ℃. Results The calibration curve was linear over the range of 0.1~20 μg/L. The LLOQ of Atorvastatin in plasma was 0.1 μg/L. The extracted recovery was>70%. The intra-and inter-day RSD were<15%. Conclusion The method is sensitive, simple and accurate to determinate Atorvastatin plasma concentration and suitable to study phase I clinical test of Atorvastatin.
  • [1] Plosker GL, Wagstaff AJ. Fluvastatin: a review of its pharmacology and use in the management og hypercholesterolaemia[J]. Drμgs, 1996, 51: 433.
    [2] Naoumova RP, Marais AD, Mountney J,et al. Plasma mevalonic acid, an index of cholesterol synthesis in vivo and responsiveness to HMG-CoA reductase inhibitors in familial hypercholesterolaemia[J]. Atherosclerosis, 1996, 119: 203.
    [3] 胡晓玲,李环德. UPLC-MS/MS法测定健康人血浆中的阿托伐他汀浓度及药物代谢动力学研究[J].中南药学,2008,6(4):400.
    [4] 董 婧, 陈西敬, 宋 捷, 等. 阿托伐他汀大鼠体内药动学及肠肝循环研究[J]. 中国药科大学学报,2008,39(1):55.
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Determination of Atorvastatin in human plasma by LC-MS-MS

  • Department of Pharmacy,General Hospital of Beijing Military Command of PLA,Beijing 100700,China
  • Received Date: 2010-05-18
  • Rev Recd Date: 2010-09-09

Abstract: Objective To establish a method for determining Atorvastatin in human plasma by LC-MS-MS. Methods Atorvastatin was extracted for determination by LC-MS-MS. Analytical column was Thermo BioBasic-C8(2.1 mm×100 mm,5 μm). The mobile phase was acetonitrile(0.1%acid):water(0.1%acid)=70:30. Mass spectrum conditions was ESI performing in the SRM mode using target ions m/z 558→278(30EV)(Atorvastatin ), m/z 269→106(22 EV)(tolbutamide), SP 3 500 KV, SGP 10 Arb, AGP 15 Arb, TEM 314 ℃. Results The calibration curve was linear over the range of 0.1~20 μg/L. The LLOQ of Atorvastatin in plasma was 0.1 μg/L. The extracted recovery was>70%. The intra-and inter-day RSD were<15%. Conclusion The method is sensitive, simple and accurate to determinate Atorvastatin plasma concentration and suitable to study phase I clinical test of Atorvastatin.

Jiang Nan, Yang Yong-Ge, Xu Xue-Ting, Zhao Gang-Tao. Determination of Atorvastatin in human plasma by LC-MS-MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 15-16,70.
Citation: Jiang Nan, Yang Yong-Ge, Xu Xue-Ting, Zhao Gang-Tao. Determination of Atorvastatin in human plasma by LC-MS-MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 15-16,70.
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