Determination of Atorvastatin in human plasma by LC-MS-MS
- Received Date: 2010-05-18
- Rev Recd Date: 2010-09-09
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Key words:
- Atorvastatin /
- plasma concentration /
- LC-MS-MS
Abstract: Objective To establish a method for determining Atorvastatin in human plasma by LC-MS-MS. Methods Atorvastatin was extracted for determination by LC-MS-MS. Analytical column was Thermo BioBasic-C8(2.1 mm×100 mm,5 μm). The mobile phase was acetonitrile(0.1%acid):water(0.1%acid)=70:30. Mass spectrum conditions was ESI performing in the SRM mode using target ions m/z 558→278(30EV)(Atorvastatin ), m/z 269→106(22 EV)(tolbutamide), SP 3 500 KV, SGP 10 Arb, AGP 15 Arb, TEM 314 ℃. Results The calibration curve was linear over the range of 0.1~20 μg/L. The LLOQ of Atorvastatin in plasma was 0.1 μg/L. The extracted recovery was>70%. The intra-and inter-day RSD were<15%. Conclusion The method is sensitive, simple and accurate to determinate Atorvastatin plasma concentration and suitable to study phase I clinical test of Atorvastatin.
Citation: | Jiang Nan, Yang Yong-Ge, Xu Xue-Ting, Zhao Gang-Tao. Determination of Atorvastatin in human plasma by LC-MS-MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 15-16,70. |