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ZHANG Wei, GAO Jing, ZHANG Min, WANG Xiao-Yu, HOU Xue-Mei, GAO Shen. Bio-efficacy of seven skin-lighteners on dermal melanocytes in vitro[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 24-26,40.
Citation: ZHANG Wei, GAO Jing, ZHANG Min, WANG Xiao-Yu, HOU Xue-Mei, GAO Shen. Bio-efficacy of seven skin-lighteners on dermal melanocytes in vitro[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 24-26,40.

Bio-efficacy of seven skin-lighteners on dermal melanocytes in vitro

  • Received Date: 2010-07-05
  • Rev Recd Date: 2010-12-17
  • Objective To investigate the bio-efficacy of seven skin-lighteners on melanocytes in vitro. Methods After B-16 melanoma cells were treated with skin-lightener, the tyrosinase activity, the melanin content and cell viability were measured by utilization of L-Dopa as the substrate, colorimetric analysis, and MTT assay, respectively. Results each of the skin-lighteners showed a concentration-dependent reduction in tyrosinase activity and the melanin content and partially inhibited the cell viability. Conclusion all of the seven skin-lighteners could inhibit the melanocytes to produce melanin.
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    [8] 杜亚威,杨文玲,刘红梅.维生素C磷酸酯衍生物的制备及其在化妆品中的应用[J].香料香精化妆品,2007,(1):26.
    [9] Zhong SM, Wu Y, SOO-MI Ahn, et al. Depigmentation of Melanocytes by the Treatment of Extracts from Traditional Chinese Herbs: a Cell Culture Assay[J].Biol. Pharm. Bull., 2006, 29(9): 1947.
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Bio-efficacy of seven skin-lighteners on dermal melanocytes in vitro

Abstract: Objective To investigate the bio-efficacy of seven skin-lighteners on melanocytes in vitro. Methods After B-16 melanoma cells were treated with skin-lightener, the tyrosinase activity, the melanin content and cell viability were measured by utilization of L-Dopa as the substrate, colorimetric analysis, and MTT assay, respectively. Results each of the skin-lighteners showed a concentration-dependent reduction in tyrosinase activity and the melanin content and partially inhibited the cell viability. Conclusion all of the seven skin-lighteners could inhibit the melanocytes to produce melanin.

ZHANG Wei, GAO Jing, ZHANG Min, WANG Xiao-Yu, HOU Xue-Mei, GAO Shen. Bio-efficacy of seven skin-lighteners on dermal melanocytes in vitro[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 24-26,40.
Citation: ZHANG Wei, GAO Jing, ZHANG Min, WANG Xiao-Yu, HOU Xue-Mei, GAO Shen. Bio-efficacy of seven skin-lighteners on dermal melanocytes in vitro[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 24-26,40.
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