Determination of a flavonol glycoside in Equisetum hiemale by RP-HPLC

LI Yan; ZHU Jin-Hui; LI Dan; GUO Cheng

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Objective To establish a RP-HPLC method for determination of a flavonol glycoside in Equisetum hiemale. Methods The separation was performed on Calesil ODS-100(250 mm×4.6 mm,5 μm). Mobile phase consisted of methanol-water (45:55) with flow rate of 1.0 ml/min and detection wavelength of 254 nm. The column temperature was 30 ℃ and injection volume was 10 μl. Results The linear range of the flavonol glycoside was 0.003 12~0.104 mg/ml with average recovery of 100.2% ( RSD=1.74%, n=9 ). The method was applied to samples of Equisetum hiemale from different locations. Conclusion The established method was proved to be simple, reproducible, accurate and could be used to evaluate the quality of Equisetum hiemale.

Determination of a flavonol glycoside in Equisetum hiemale by RP-HPLC

Department of Pharmacy, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China

Received Date: 2010-07-13

Rev Recd Date: 2009-08-31

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Abstract: Objective To establish a RP-HPLC method for determination of a flavonol glycoside in Equisetum hiemale. Methods The separation was performed on Calesil ODS-100(250 mm×4.6 mm,5 μm). Mobile phase consisted of methanol-water (45:55) with flow rate of 1.0 ml/min and detection wavelength of 254 nm. The column temperature was 30 ℃ and injection volume was 10 μl. Results The linear range of the flavonol glycoside was 0.003 12~0.104 mg/ml with average recovery of 100.2% ( RSD=1.74%, n=9 ). The method was applied to samples of Equisetum hiemale from different locations. Conclusion The established method was proved to be simple, reproducible, accurate and could be used to evaluate the quality of Equisetum hiemale.

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Reference (10)

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Determination of a flavonol glycoside in Equisetum hiemale by RP-HPLC

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