Determination of sodium Danshensu in rat plasma by HPLC-MS/MS

WANG Jing; JIN Lei; XIONG Li-yan; WANG Ting-fang; ZHENG Li-ping; ZHANG Ning; ZOU Hao; ZHANG Chuan

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Objective To estiblish a method of determining sodium Danshensu in rat plasma by HPLC-MS/MS. Methods sodium Danshensu was extrated for determination by HPLC-MS/MS. Analytical column was Diamonsil C₁₈(4.6 mm×200 mm ,5 μm). The mobile phase was methanolwater (0.01‰ formic acid )= 8020. Mass spectrum conditions were ESI performing in the MRM mode using target ions m/z 197→135 (Sodium Danshensu), m/z 253→209 (ketoprofen). Results The calibration curve was liner over the range of 10.6~1 060 ng/ml. The LLOQ of sodium Danshensu in plasma was 10.6 ng/ml. The intra-day and inter-day RSD were<7.8%. The extracted recovery was ranged from 99% to 102%. Conclusion The method was sensitive, rapid, simple and accurate to sodium Danshensu plasma concertration and suitable to study preclinical test of sodium Danshensu.

Determination of sodium Danshensu in rat plasma by HPLC-MS/MS

1. New drug research center, School of Pharmcy, Second military medical university, Shanghai 200433, China

2. New drug research center, School of Pharmcy, Second military medical university, Shanghai 200433, China;School of Pharmacy, Fujian university of traditional Chinese medcine , Fuzhou 350108, China

3. Central Hospital of Xuhui District, Shanghai 200031, China

Received Date: 2011-03-01

Rev Recd Date: 2011-03-07

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Abstract: Objective To estiblish a method of determining sodium Danshensu in rat plasma by HPLC-MS/MS. Methods sodium Danshensu was extrated for determination by HPLC-MS/MS. Analytical column was Diamonsil C₁₈(4.6 mm×200 mm ,5 μm). The mobile phase was methanolwater (0.01‰ formic acid )= 8020. Mass spectrum conditions were ESI performing in the MRM mode using target ions m/z 197→135 (Sodium Danshensu), m/z 253→209 (ketoprofen). Results The calibration curve was liner over the range of 10.6~1 060 ng/ml. The LLOQ of sodium Danshensu in plasma was 10.6 ng/ml. The intra-day and inter-day RSD were<7.8%. The extracted recovery was ranged from 99% to 102%. Conclusion The method was sensitive, rapid, simple and accurate to sodium Danshensu plasma concertration and suitable to study preclinical test of sodium Danshensu.

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[1]	Ji XY, Tan BK, Zhu YZ. Salvia miltiorrhiza and ischemic diseases[J]. Acta Pharmacol Shin, 2000, 21(12): 1089.
[2]	Chen KJ. Certain progress in the treatment of coronary heart disease with traditional medicinal plants in China[J]. Am J Chin Med, 1981, 9(3): 193.
[3]	吕亚青.丹参的化学成分及临床应用进展[J]. 中国药房, 2007, 18(12): 917.
[4]	Zeng G, Xiao H, Liu J, et al. Identification of phenolic constituents in Radix Salvia miltiorrhizae by liquid chromatography/electrospray ionization mass spectrometry[J]. Rapid Commun Mass Spectrom, 2006, 20(3): 499.
[5]	Huang YS, Zhang JT. Antioxidative effect of three watersoluble components isolated from Salvia miltiorrhiza in vitro[J]. Acta Pharm Sin, 1992, 27(2): 96.
[6]	Zhang LJ, Chen L, Lu Y, et al. Danshensu has anti-tumor activity in B16F10 melanoma by inhibiting angiogenesis and tumor cell invasion[J]. Int J Pharm, 2010, 643(2-3): 195.
[7]	Zhao GR, Zhang HM, Ye TX, et al. Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B[J]. Food Chem Toxicol, 2008, 46(1): 73.
[8]	金昔陆, 陈滨凌, 吴卫江,等. 8 种丹参素衍生物对兔血小板聚集性的影响[J]. 上海医科大学学报, 2000, 27(3): 181.
[9]	赵新峰, 祝忠民, 刘爱芳, 等. 柱切换法在复方丹参滴丸中丹参素药代动力学研究中的应用[J]. 中成药, 2004, 26(6): 490.
[10]	Li XL, Li XR, Wang LJ, et al. Simultaneous determination of danshensu, ferulic acid, cryptotanshinone and tanshinone IIA in rabbit plasma by HPLC and their pharmacokinetic application in danxiongfang[J]. J Pharmaceut Biomed, 2007, 44(5): 1106.
[11]	智红英, 钟大放, 马小菊, 等. 高效液相色谱法测定犬血浆中丹参素钠[J]. 中草药, 2009, 40(6): 886.
[12]	蒋亚萍, 胡小鹰. 丹七注射液中丹参素钠在Beagle犬体内药代动力学及生物利用度研究[J]. 安徽医药, 2009, 13(1): 17.
[13]	胡珊珊, 刘洁, 刘文静, 等. HPLC-FLD法测定丹参素在家兔组织中的分布[J]. 第四军医大学学报, 2008, 29(7): 670.
[14]	张雪, 梅文静, 刘文娜, 等. 高效液相色谱二极管阵列检测法同时测定丹参滴注液中四种水溶性成分的含量[J]. 分析化学学报, 2010, 29(1): 109.

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Determination of sodium Danshensu in rat plasma by HPLC-MS/MS

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