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MA Jun, FAN Peng-cheng, REN Jun, JIA Zheng-ping. Detection of donepezil from serum by on-line sample preparation RAM-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(2): 122-124.
Citation: MA Jun, FAN Peng-cheng, REN Jun, JIA Zheng-ping. Detection of donepezil from serum by on-line sample preparation RAM-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(2): 122-124.

Detection of donepezil from serum by on-line sample preparation RAM-HPLC

  • Received Date: 2009-12-10
  • Rev Recd Date: 2010-10-02
  • Objective To establish a sample preparation restricted access material (RAM) HPLC method for the quantification of donepezil in human serum. Method The sample was prepocessed by LiChrospher ADS C18 (25 mm × 4 mm,25 μm) as pre-column and analyzed by Microsorb-MV C18 (150 mm × 4.6 mm,4 μm ) as analytical column. Acetonitrile to water (991, v/v) was flushing mobile phase with flow rate of 2 ml/min. The mixture of phosphate buffer (pH 4.6, 0.02 M), acetonitril and triethylamine (65350.1, v/v/v) was analytical mobile phase with flow rate of 1 ml/min. The detection wave was 315 nm. Results The linear range was from 0.02 to 1.0 μg/ml (r=0.999 5). The RSDs of intra-day and inter-day were less than 7%, the recoveries were between 87.9%~91.1%. Conclusion The method is accurate, stable and sensitive which could be used to study the pharmacodynamics and bioavailabilty of donepezil.
  • [1] Rogers SL, Farlow MR, Doody RS, et al. A 24-week, double-blind, placebo-controlled trial of donepezll in patients with Alzheimer 's disease[J]. Neurology, 1998, 50: 136.
    [2] Norio YF, Furuya R, Takahata T, et al. Determination of donepezil, an acetylcholinesterase inhibitor, in human plasma by high-performance liquid chromatography with ultraviolet absorbance detection[J]. J Chromatogr B, 2002, 768: 261.
    [3] Radwan MA, Abdine HH, Al-Quadeb BT, et al. Stereoselective HPLC assay of donepezil enantiomers with UV detection and its application to pharmacokinetics in rats[J]. J Chromatogr B, 2006, 830: 114.
    [4] Nakashima K, Itoh K, Kono M, et al. Determination of donepezil hydrochloride in human and rat plasma, blood and brain microdialysates by HPLC with a short C30 column[J]. J Pharm Biomed Anal, 2006, 41:201.
    [5] Souverain S, Rudaz S, Veuthey JL, et al. Restricted access materials and large particle supports for on-line sample preparation: an attractive approach for biological fluids analysis[J]. J Chromatogr B, 2004, 801:141.
    [6] Chiap P, Piette M, Evrard B, et al. Automated method for the determination of a new matrix metalloproteinase inhibitor in ovine plasma and serum by coupling of restricted access material for on-line sample clean-up to liquid chromatography[J]. J Chromatogr B, 2005, 817:109.
    [7] Rbeida O, Chiap P, Lubda D, et al. Development and validation of a fully automated LC method for the determination of cloxacillin in human plasma using anion exchange restricted access material for sample clean-up[J]. J Pharm Biomed Anal, 2005, 36: 961.
    [8] Vintiloiu A, Mullett WM, Papp R, et al. Combining restricted access material (RAM) and turbulent flow for the rapid on-line extraction of the cyclooxygenase-2 inhibitor rofecoxib in plasma samples[J]. J Chromatogr A, 2005, 1082:150.
    [9] Musteata FM, Walles M, Pawliszyn J. Fast assay of angiotensin 1 from whole blood by cation-exchange restricted-access solid-phase microextraction[J]. Anal Chim Acta, 2005, 537: 231.
    [10] Lambert JP, Mullett WM, Kwong E, et al. Stir bar sorptive extraction based on restricted access material for the direct extraction of caffeine and metabolites in biological fluids[J]. J Chromatogr A, 2005, 1075: 43.
    [11] Neto AJ, Rodrigues JC, Fernandes C, et al. Automated microcolumn-switching system for drug analysis by direct injection of human plasma[J]. J Chromatogr A, 2006, 1105:71.
    [12] Ortelli D, Rudaz S, Souverain S, et al. Restricted access materials for fast analysis of methadone in serum with liquid chromatography-mass spectrometry[J]. J Sep Sci, 2002, 25:222.
    [13] 樊鹏程, 马 骏, 贾正平, 等. 在线柱切换RP-HPLC测定人血清中卡马西平浓度[J].药物分析杂志, 2008, 28(12): 2033.
    [14] Yamamoto E, Igarashi H, Sato Y, et al. Reliable on-line sample preparation of basic compounds from plasma using a reversed phase restricted access media in column-switching LC[J]. J Pharm Biomed Anal, 2006, 42:587.
    [15] Pappa H, Farru R, Vilanova PO, et al. A new HPLC method to determine Donepezil hydrochloride in tablets[J]. J Pharm Biomed Anal, 2002, 27:177.
    [16] Asakawa Y, Ozawa C, Osada K, et al. Reduction of carry-over in column-switching HPLC/MS system with automated system washing procedure for highly sensitive direct analysis of donepezil in dog plasma[J]. J Pharm Biomed Anal, 2007, 43:683.
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Detection of donepezil from serum by on-line sample preparation RAM-HPLC

Abstract: Objective To establish a sample preparation restricted access material (RAM) HPLC method for the quantification of donepezil in human serum. Method The sample was prepocessed by LiChrospher ADS C18 (25 mm × 4 mm,25 μm) as pre-column and analyzed by Microsorb-MV C18 (150 mm × 4.6 mm,4 μm ) as analytical column. Acetonitrile to water (991, v/v) was flushing mobile phase with flow rate of 2 ml/min. The mixture of phosphate buffer (pH 4.6, 0.02 M), acetonitril and triethylamine (65350.1, v/v/v) was analytical mobile phase with flow rate of 1 ml/min. The detection wave was 315 nm. Results The linear range was from 0.02 to 1.0 μg/ml (r=0.999 5). The RSDs of intra-day and inter-day were less than 7%, the recoveries were between 87.9%~91.1%. Conclusion The method is accurate, stable and sensitive which could be used to study the pharmacodynamics and bioavailabilty of donepezil.

MA Jun, FAN Peng-cheng, REN Jun, JIA Zheng-ping. Detection of donepezil from serum by on-line sample preparation RAM-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(2): 122-124.
Citation: MA Jun, FAN Peng-cheng, REN Jun, JIA Zheng-ping. Detection of donepezil from serum by on-line sample preparation RAM-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(2): 122-124.
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