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Volume 29 Issue 2
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2011  >  29(2): 128-130

WANG Zong-chun, ZHOU Yan-an, ZHANG Hong. Establishment of bacterial endotoxins test in extracts injection from rabbit skin inflamed by vaccinia virus[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(2): 128-130.
Citation: WANG Zong-chun, ZHOU Yan-an, ZHANG Hong. Establishment of bacterial endotoxins test in extracts injection from rabbit skin inflamed by vaccinia virus[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(2): 128-130.
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Establishment of bacterial endotoxins test in extracts injection from rabbit skin inflamed by vaccinia virus

  • Department of Pharmacy, Renmin Hospital of Wuhan University, Wuhan 430060,China

  • Received Date: 2010-08-12
  • Rev Recd Date: 2010-11-21
  • Objective To establish a method for the detection of bacterial endotoxin in extracts injection from rabbit skin inflamed by vaccinia virus. Methods The interference test was carried out according to the guideline and procedure specified in appendix Ⅺ E, volume two, chinese pharmacopoeia 2010 edition. Results By using tachypleus amebocyte lysates which sensitivity was 0.5 EU/ml, the sample did not interfere with the bacterial endotoxins test when it was diluted 100 times. Conclusion The established method of bacterial endotoxins test for extracts injection from rabbit skin inflamed by vaccinia virus was feasible.
  • [1] 中国药典2010版. 二部[S]. 2010. 附录99.
    [2] 中国药品生物制品检定所. 中国药品检验标准操作规范[M]. 北京: 中国医药科技出版社,2005: 287.
    [3] 高 锦,付 聪,嵇 扬,等. 复方氯解磷定注射液细菌内毒素的检查方法[J]. 药学实践杂志,2008,26(2):133.
    [4] 胡宇驰,杨赟昀,朱春玥,等. 醋酸戈舍瑞林缓释植入剂细菌内毒素检查方法学研究[J]. 药学实践杂志,2010,28(1):36.
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Establishment of bacterial endotoxins test in extracts injection from rabbit skin inflamed by vaccinia virus

  • Department of Pharmacy, Renmin Hospital of Wuhan University, Wuhan 430060,China
  • Received Date: 2010-08-12
  • Rev Recd Date: 2010-11-21

Abstract: Objective To establish a method for the detection of bacterial endotoxin in extracts injection from rabbit skin inflamed by vaccinia virus. Methods The interference test was carried out according to the guideline and procedure specified in appendix Ⅺ E, volume two, chinese pharmacopoeia 2010 edition. Results By using tachypleus amebocyte lysates which sensitivity was 0.5 EU/ml, the sample did not interfere with the bacterial endotoxins test when it was diluted 100 times. Conclusion The established method of bacterial endotoxins test for extracts injection from rabbit skin inflamed by vaccinia virus was feasible.

WANG Zong-chun, ZHOU Yan-an, ZHANG Hong. Establishment of bacterial endotoxins test in extracts injection from rabbit skin inflamed by vaccinia virus[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(2): 128-130.
Citation: WANG Zong-chun, ZHOU Yan-an, ZHANG Hong. Establishment of bacterial endotoxins test in extracts injection from rabbit skin inflamed by vaccinia virus[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(2): 128-130.
shu

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