Effect of dissolved oxygen on high density fermentation of E.coli in producing rhG-CSF

CEN Yi; YANG Feng; TONG Yong

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Objective To study the density of the dissolved oxygen(DO) on fermentation of E.coli in producing rhG-CSF. Methods Pure oxygen was used to control DO precisely and to observe the growth of engineered bacteria, the lost of plasmids ,the accumulation of acetic acid from different DO density. The optimum density of DO for fermentation was determined. Results 25% in growth phase and 30% after induction was optimum density of DO for rhG-CSF expression and engineered bacteria growth. Under these conditions, the wet cell weight reached(79.6±2.1) g/L and the expression of rhG-CSF was(40.2±1.2)%. Conclusions The growth of engineered bacteria and the expression of rhG-CSF were influenced by the density of dissolved oxygen significantly.

Effect of dissolved oxygen on high density fermentation of E.coli in producing rhG-CSF

1. Shanghai Sunwaybio Company, Shanghai 201206,China

2. Department of Inorganic Chemistry,School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received Date: 2011-01-22

Rev Recd Date: 2011-04-22

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Abstract: Objective To study the density of the dissolved oxygen(DO) on fermentation of E.coli in producing rhG-CSF. Methods Pure oxygen was used to control DO precisely and to observe the growth of engineered bacteria, the lost of plasmids ,the accumulation of acetic acid from different DO density. The optimum density of DO for fermentation was determined. Results 25% in growth phase and 30% after induction was optimum density of DO for rhG-CSF expression and engineered bacteria growth. Under these conditions, the wet cell weight reached(79.6±2.1) g/L and the expression of rhG-CSF was(40.2±1.2)%. Conclusions The growth of engineered bacteria and the expression of rhG-CSF were influenced by the density of dissolved oxygen significantly.

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Effect of dissolved oxygen on high density fermentation of E.coli in producing rhG-CSF

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