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ZHOU Heng, LIN Huan-bing, ZHUO Ye-ye, Bi Bing-tian, Li Guo-xiong, XU Jiang-ping. Effects of grape seed procyanidins on spatial learning and memory in vascular dementia model of rats[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 208-211.
Citation:
ZHOU Heng, LIN Huan-bing, ZHUO Ye-ye, Bi Bing-tian, Li Guo-xiong, XU Jiang-ping. Effects of grape seed procyanidins on spatial learning and memory in vascular dementia model of rats[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 208-211.
Effects of grape seed procyanidins on spatial learning and memory in vascular dementia model of rats
Department of Neuropsychopharmacology, School of Pharmacy, Southern Medical University, Guangzhou 510515, China
2.
Center for evaluation & certification, Guangdong food and drug administration, Guangzhou 510080, China
Received Date: 2011-01-07
Rev Recd Date:
2011-03-13
Abstract
Objective To investigate the effects and possible mechanisms of procyanidins, a polyphenol extracted from grape seeds, on spatial learning and memory in rats model of vascular dementia(VD). Methods Modified Pulsinelli's 4-vessel occlusion (4-VO) method was used for replicating vascular dementia in rats. The male Wistar rats were divided equally into 5 experimental groups: in one the rats were subjected to sham-operated, and in the other four the rats was subjected to the 4-VO surgery followed by oral administration of vehicle, Ginkgo leaf extract(24 mg/kg), procyanidins(50 or 150 mg/kg) respectively. The spatial learning and memory performance was recorded as the acquisition trial and probe trial in the Morris water maze. The levels of malondialdehyde(MDA), glutathione(GSH), total antioxidant capacity(T-AOC) and the activity of superoxidedismutase(SOD) in the brain tissue were determined by colorimetric Assay Kit. The gene expression of Bax and Bcl-2 in the rat hippocampus was detected by real-time PCR. Results The vascular dementia rats treated with procyanidins showed decreased escaped latency during acquisition training, especially at the dose of 150 mg/kg, procyanidins also increased exploration time and frequency of crossing in the target quadrant during probe trial. The administration of procyanidins induced increase in GSH, T-AOC contents and SOD activities with lowering MDA levels in the brain. In addition, procyanidins upregulated the Bcl-2/Bax ratio of mRNA expression in the rat hippocampus. Conclusion Procyanidins could have potentials for improving the spatial cognition with VD,which was possibly due to the antioxidative and anti-apoptotic effects of polyphenol.
Zhang ZX, Zahner GEP, Roman GC, et al. Dementia subtypes in China: prevalence in Beijing, Xian, Shanghai, and Chengdu[J]. Arch Neurol, 2005, 62(3): 447.
Hooge RD, Deyn PPDe. Applications of the Morris water maze in the study of learning and memory[J]. Brain Research Reviews, 2001, 36(1): 60.
[8]
Bagchi D, Bagchi M, Stohs SJ, et al. Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention[J]. Toxicology, 2000, 148(2-3): 187.
Abstract: Objective To investigate the effects and possible mechanisms of procyanidins, a polyphenol extracted from grape seeds, on spatial learning and memory in rats model of vascular dementia(VD). Methods Modified Pulsinelli's 4-vessel occlusion (4-VO) method was used for replicating vascular dementia in rats. The male Wistar rats were divided equally into 5 experimental groups: in one the rats were subjected to sham-operated, and in the other four the rats was subjected to the 4-VO surgery followed by oral administration of vehicle, Ginkgo leaf extract(24 mg/kg), procyanidins(50 or 150 mg/kg) respectively. The spatial learning and memory performance was recorded as the acquisition trial and probe trial in the Morris water maze. The levels of malondialdehyde(MDA), glutathione(GSH), total antioxidant capacity(T-AOC) and the activity of superoxidedismutase(SOD) in the brain tissue were determined by colorimetric Assay Kit. The gene expression of Bax and Bcl-2 in the rat hippocampus was detected by real-time PCR. Results The vascular dementia rats treated with procyanidins showed decreased escaped latency during acquisition training, especially at the dose of 150 mg/kg, procyanidins also increased exploration time and frequency of crossing in the target quadrant during probe trial. The administration of procyanidins induced increase in GSH, T-AOC contents and SOD activities with lowering MDA levels in the brain. In addition, procyanidins upregulated the Bcl-2/Bax ratio of mRNA expression in the rat hippocampus. Conclusion Procyanidins could have potentials for improving the spatial cognition with VD,which was possibly due to the antioxidative and anti-apoptotic effects of polyphenol.
ZHOU Heng, LIN Huan-bing, ZHUO Ye-ye, Bi Bing-tian, Li Guo-xiong, XU Jiang-ping. Effects of grape seed procyanidins on spatial learning and memory in vascular dementia model of rats[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 208-211.
Citation:
ZHOU Heng, LIN Huan-bing, ZHUO Ye-ye, Bi Bing-tian, Li Guo-xiong, XU Jiang-ping. Effects of grape seed procyanidins on spatial learning and memory in vascular dementia model of rats[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 208-211.
Zhang ZX, Zahner GEP, Roman GC, et al. Dementia subtypes in China: prevalence in Beijing, Xian, Shanghai, and Chengdu[J]. Arch Neurol, 2005, 62(3): 447.
Hooge RD, Deyn PPDe. Applications of the Morris water maze in the study of learning and memory[J]. Brain Research Reviews, 2001, 36(1): 60.
[8]
Bagchi D, Bagchi M, Stohs SJ, et al. Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention[J]. Toxicology, 2000, 148(2-3): 187.
ZHOU Heng, LIN Huan-bing, ZHUO Ye-ye, Bi Bing-tian, Li Guo-xiong, XU Jiang-ping. Effects of grape seed procyanidins on spatial learning and memory in vascular dementia model of rats[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 208-211.
ZHOU Heng, LIN Huan-bing, ZHUO Ye-ye, Bi Bing-tian, Li Guo-xiong, XU Jiang-ping. Effects of grape seed procyanidins on spatial learning and memory in vascular dementia model of rats[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 208-211.