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Volume 29 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2011  >  29(4): 269-271

WANG Zhi-jun, YOU Ben-ming, SUN Hua-jun, SONG Hong-jie, LIU Ji-yong. Determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(4): 269-271.
Citation: WANG Zhi-jun, YOU Ben-ming, SUN Hua-jun, SONG Hong-jie, LIU Ji-yong. Determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(4): 269-271.
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Determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion by RP-HPLC

  • Department of Pharmacy, Changhai Hospital,Second Military Medical University, Shanghai 200433, China

  • Received Date: 2011-06-10
  • Rev Recd Date: 2011-06-30
  • Objective To develop a RP-HPLC method for the determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion. Methods The separation was performed on a Kromasil ODS C18 analytical column (150 mm×4.6 mm, 5μm) with the mobile phase consisting of acetonitrile-water(90：10).The detection wavelength was 210 nm.The column temperature was room temperature. The flow rate was 1.0 ml/min and the sample injection volume was 20 μl. Results Wilforlide presented a good line relationship in the range of 2.0~30.0 μg/ml(r=0.999 9). The average recovery rate was (100.16±0.56)％(n=9).The average contents of Wilforlide in three batch samples were(98.92±0.92)%. Conclusion It was a sensitive, selective and reliable method for the determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion.
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    [3] 寇 欣.微乳给药系统的研究进展[J]. 天津药学, 2005, 17(6): 52.
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    [6] 王高森,侯世祥,毛生俊等. 丹参酮ⅡA微乳的制备与质量评价[J]. 华西药学杂志, 2007, 22(3): 279.
    [7] 谢 谊,王实强,刘可柔. 反相高效液相色谱法测定雷公藤多甙片中雷公藤酯甲的含量研究[J]. 湖南中医杂志,2007,23(1):79.
    [8] 刘法千,鲍立曾,熊仕强. HPLC法测定雷公藤多苷中雷公藤内酯甲的含量[J].中国药品标准,2007,8(4):38.
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Determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion by RP-HPLC

  • Department of Pharmacy, Changhai Hospital,Second Military Medical University, Shanghai 200433, China
  • Received Date: 2011-06-10
  • Rev Recd Date: 2011-06-30

Abstract: Objective To develop a RP-HPLC method for the determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion. Methods The separation was performed on a Kromasil ODS C18 analytical column (150 mm×4.6 mm, 5μm) with the mobile phase consisting of acetonitrile-water(90：10).The detection wavelength was 210 nm.The column temperature was room temperature. The flow rate was 1.0 ml/min and the sample injection volume was 20 μl. Results Wilforlide presented a good line relationship in the range of 2.0~30.0 μg/ml(r=0.999 9). The average recovery rate was (100.16±0.56)％(n=9).The average contents of Wilforlide in three batch samples were(98.92±0.92)%. Conclusion It was a sensitive, selective and reliable method for the determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion.

WANG Zhi-jun, YOU Ben-ming, SUN Hua-jun, SONG Hong-jie, LIU Ji-yong. Determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(4): 269-271.
Citation: WANG Zhi-jun, YOU Ben-ming, SUN Hua-jun, SONG Hong-jie, LIU Ji-yong. Determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(4): 269-271.
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