Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 29 Issue 4
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2011  >  29(4): 269-271

WANG Zhi-jun, YOU Ben-ming, SUN Hua-jun, SONG Hong-jie, LIU Ji-yong. Determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(4): 269-271.
Citation: WANG Zhi-jun, YOU Ben-ming, SUN Hua-jun, SONG Hong-jie, LIU Ji-yong. Determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(4): 269-271.
shu

Determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion by RP-HPLC

  • Department of Pharmacy, Changhai Hospital,Second Military Medical University, Shanghai 200433, China

  • Received Date: 2011-06-10
  • Rev Recd Date: 2011-06-30
  • Objective To develop a RP-HPLC method for the determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion. Methods The separation was performed on a Kromasil ODS C18 analytical column (150 mm×4.6 mm, 5μm) with the mobile phase consisting of acetonitrile-water(90：10).The detection wavelength was 210 nm.The column temperature was room temperature. The flow rate was 1.0 ml/min and the sample injection volume was 20 μl. Results Wilforlide presented a good line relationship in the range of 2.0~30.0 μg/ml(r=0.999 9). The average recovery rate was (100.16±0.56)％(n=9).The average contents of Wilforlide in three batch samples were(98.92±0.92)%. Conclusion It was a sensitive, selective and reliable method for the determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion.
  • [1] 徐央丽.雷公藤的研究进展[J].现代中西医结合杂志,2008,17(12):1914.
    [2] 金美子.雷公藤研究进展[J].中国社区医师,2007,21:34.
    [3] 寇 欣.微乳给药系统的研究进展[J]. 天津药学, 2005, 17(6): 52.
    [4] Peltola S, Saarinen-Savolainen P. Microemulsions for topical delivery of estradiol[J]. Int J Pharm, 2003, 254(2): 99.
    [5] Rhee YS, Choi JG. Transdermal delivery of ketoprofen using microemulsions[J]. Int J Pharm, 2001, 228(1-2): 161.
    [6] 王高森,侯世祥,毛生俊等. 丹参酮ⅡA微乳的制备与质量评价[J]. 华西药学杂志, 2007, 22(3): 279.
    [7] 谢 谊,王实强,刘可柔. 反相高效液相色谱法测定雷公藤多甙片中雷公藤酯甲的含量研究[J]. 湖南中医杂志,2007,23(1):79.
    [8] 刘法千,鲍立曾,熊仕强. HPLC法测定雷公藤多苷中雷公藤内酯甲的含量[J].中国药品标准,2007,8(4):38.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(2255) PDF downloads(320) Cited by()

Related
Proportional views

Determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion by RP-HPLC

  • Department of Pharmacy, Changhai Hospital,Second Military Medical University, Shanghai 200433, China
  • Received Date: 2011-06-10
  • Rev Recd Date: 2011-06-30

Abstract: Objective To develop a RP-HPLC method for the determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion. Methods The separation was performed on a Kromasil ODS C18 analytical column (150 mm×4.6 mm, 5μm) with the mobile phase consisting of acetonitrile-water(90：10).The detection wavelength was 210 nm.The column temperature was room temperature. The flow rate was 1.0 ml/min and the sample injection volume was 20 μl. Results Wilforlide presented a good line relationship in the range of 2.0~30.0 μg/ml(r=0.999 9). The average recovery rate was (100.16±0.56)％(n=9).The average contents of Wilforlide in three batch samples were(98.92±0.92)%. Conclusion It was a sensitive, selective and reliable method for the determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion.

WANG Zhi-jun, YOU Ben-ming, SUN Hua-jun, SONG Hong-jie, LIU Ji-yong. Determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(4): 269-271.
Citation: WANG Zhi-jun, YOU Ben-ming, SUN Hua-jun, SONG Hong-jie, LIU Ji-yong. Determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(4): 269-271.
shu

    HTML

Reference (8)

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return