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CAO Yan, JING Jing, LV Di-ya, ZHU Zhen-yu, ZHANG Guo-qing, CHAI Yi-feng. Study on effective components from roots of Aconitum carmichaeli through cell membrane chromatography and HPLC-TOF/MS method[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 339-341,349.
Citation:
CAO Yan, JING Jing, LV Di-ya, ZHU Zhen-yu, ZHANG Guo-qing, CHAI Yi-feng. Study on effective components from roots of Aconitum carmichaeli through cell membrane chromatography and HPLC-TOF/MS method[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 339-341,349.
Study on effective components from roots of Aconitum carmichaeli through cell membrane chromatography and HPLC-TOF/MS method
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
2.
Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China
Received Date: 2010-03-25
Rev Recd Date:
2011-05-04
Abstract
Objective To screen the effective components from roots of Aconitum carmichaeli by a cell membrane chromatography (CMC) combining with a HPLC-TOF/MS method. Methods Screening was conducted on a rat cardiac muscle CMC column, and mobile phase was 10 mmol/L phosphate buffer with a flow rate of 0.2 ml/min. Separation was conducted on a SHISEIDO CAPCELL PAK C18 column. The mobile phase consisted of 0.1% formic acid and acetonitrile using gradient elution with a flow rate of 1.0 ml/min. Identification was conducted by using TOF/MS in positive ion mode. Results Eight potential effective constituents were determined. Conclusion The combination of CMC and HPLC-TOF/MS method had provided a new way to discover active components targeting membrane receptors from natural products.
He LC, Yang GD, Geng XD. Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface[J]. Chin Sci Bull, 1999, 44(9): 826.
[2]
He LC, Wang SC, Geng XD. Coating and fusing cell membranes onto a silica surface and their chromatographic characteristics[J]. Chromatographia, 2001, 54(1): 71.
Hu R, Zhao J, Qi LW, et al. Structural characterization and identification of C(19)-and C(20)-diterpenoid alkaloids in roots of Aconitum carmichaeli by rapid-resolution liquid chromatography coupled with time-of-flight mass spectrometry[J]. Rapid Commun Mass Sp, 2009, 23(11): 1619.
Abstract: Objective To screen the effective components from roots of Aconitum carmichaeli by a cell membrane chromatography (CMC) combining with a HPLC-TOF/MS method. Methods Screening was conducted on a rat cardiac muscle CMC column, and mobile phase was 10 mmol/L phosphate buffer with a flow rate of 0.2 ml/min. Separation was conducted on a SHISEIDO CAPCELL PAK C18 column. The mobile phase consisted of 0.1% formic acid and acetonitrile using gradient elution with a flow rate of 1.0 ml/min. Identification was conducted by using TOF/MS in positive ion mode. Results Eight potential effective constituents were determined. Conclusion The combination of CMC and HPLC-TOF/MS method had provided a new way to discover active components targeting membrane receptors from natural products.
CAO Yan, JING Jing, LV Di-ya, ZHU Zhen-yu, ZHANG Guo-qing, CHAI Yi-feng. Study on effective components from roots of Aconitum carmichaeli through cell membrane chromatography and HPLC-TOF/MS method[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 339-341,349.
Citation:
CAO Yan, JING Jing, LV Di-ya, ZHU Zhen-yu, ZHANG Guo-qing, CHAI Yi-feng. Study on effective components from roots of Aconitum carmichaeli through cell membrane chromatography and HPLC-TOF/MS method[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 339-341,349.
He LC, Yang GD, Geng XD. Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface[J]. Chin Sci Bull, 1999, 44(9): 826.
[2]
He LC, Wang SC, Geng XD. Coating and fusing cell membranes onto a silica surface and their chromatographic characteristics[J]. Chromatographia, 2001, 54(1): 71.
Hu R, Zhao J, Qi LW, et al. Structural characterization and identification of C(19)-and C(20)-diterpenoid alkaloids in roots of Aconitum carmichaeli by rapid-resolution liquid chromatography coupled with time-of-flight mass spectrometry[J]. Rapid Commun Mass Sp, 2009, 23(11): 1619.
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