JI Song-gang, WU Hai-tang, MIAO Hua-yan, WU Si, LI Xiang. Analysis on chemical constituents of ershen pill by HPLC-TOF/MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 435-438.
Citation:
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JI Song-gang, WU Hai-tang, MIAO Hua-yan, WU Si, LI Xiang. Analysis on chemical constituents of ershen pill by HPLC-TOF/MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 435-438.
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Analysis on chemical constituents of ershen pill by HPLC-TOF/MS
- 1.
Department of Pharmacy, No.401 Hospital of PLA, Qingdao 266071, China
- 2.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- 3.
No.62370 PLA troop, Beijing 100071, China
- 4.
No. 62380 PLA troop, Beijing 100071, China
- Received Date: 2011-01-18
- Rev Recd Date:
2011-10-21
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Abstract
Objective To analyze chemical constituents of ershen pill by rapid-resolution(high performance) liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS). Methods A shelloseido column(3.0 mm×100 mm,3 μm) was used to separate. The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute. The flow rate was 0.6 ml/min. TOF/MS was applied for qualitative analysis under positive ion mode. Results Under LC/MS condition, 21 major constituents in ershen pill were identified by time of flight mass spectrometry, among which, 15 constituents were from Psoralea corylifolia L,and 6 constituents were from Myristica fragrans Houtt. Conclusion A simple and reliable method using HPLC-TOF/MS was established to identify the chemical constituents of ershen pill.
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Proportional views
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