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CHEN Rui, CHU Zhong-ying, ZHANG Ji-chun, TIAN Lan. Determination of forbidden addition drugs of Diazepam,Clonazepam and Estazolam in tranquilizing mind traditional Chinese medicine by UPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 457-459.
Citation: CHEN Rui, CHU Zhong-ying, ZHANG Ji-chun, TIAN Lan. Determination of forbidden addition drugs of Diazepam,Clonazepam and Estazolam in tranquilizing mind traditional Chinese medicine by UPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 457-459.

Determination of forbidden addition drugs of Diazepam,Clonazepam and Estazolam in tranquilizing mind traditional Chinese medicine by UPLC-MS/MS

  • Received Date: 2011-05-29
  • Rev Recd Date: 2011-06-15
  • Objective To establish a specific method for determination of forbidden addition drugs of Diazepam,Clonazepan and Estazolam in tranquilizing mind Traditional Chinese Medicine. Methods Diazepam, Clonazepam and Estazolam were determined and analyzed by UPLC-MS/MS. ACQUITY BEH C18 analysis column(2.1 mm×50 mm,1.7 μm) was used with the mobile phase of 10 mmol/L ammonium acetate and acetonitrile(90:10) with gradient elution. The flow rate was 0.4 ml/min,and the detection wavelength was 254 nm.EIS ion source was used with positive ion monitoring. Results The standard curves of Diazepam,Clonazepam and Estazolam showed good linearity over the concentration rang of 109.8~878.4, 102.1~816.8,106.9~855.2 ng/ml,The detection limits were 0.06,2.5×10-3,2.8×10-3 ng,the quantitation limits were 0.19,8.2×10-3,9.2×10-3 ng,the average recoveries of three chemicals was 95.7%~103.7%. Conclution The method was specific,sensitive,easily and quickly,which could be used to detect Diazepam,Clonazepan and Estazolam in tranquilizing mind traditional Chinese medicine.
  • [1] 李欣荣,陈安珍,杨 钊.UPLC-MS/MS检测消肿止痛类中成药中违禁添加的双氯芬酸钠和氨基比林[J].药物分析杂志,2010,30(7): 1294.
    [2] 国家食品药品监督管理局药品检验补充检验方法和检验项目批准件汇编[S].批准件编号2006008:109; 2006024:139.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Determination of forbidden addition drugs of Diazepam,Clonazepam and Estazolam in tranquilizing mind traditional Chinese medicine by UPLC-MS/MS

Abstract: Objective To establish a specific method for determination of forbidden addition drugs of Diazepam,Clonazepan and Estazolam in tranquilizing mind Traditional Chinese Medicine. Methods Diazepam, Clonazepam and Estazolam were determined and analyzed by UPLC-MS/MS. ACQUITY BEH C18 analysis column(2.1 mm×50 mm,1.7 μm) was used with the mobile phase of 10 mmol/L ammonium acetate and acetonitrile(90:10) with gradient elution. The flow rate was 0.4 ml/min,and the detection wavelength was 254 nm.EIS ion source was used with positive ion monitoring. Results The standard curves of Diazepam,Clonazepam and Estazolam showed good linearity over the concentration rang of 109.8~878.4, 102.1~816.8,106.9~855.2 ng/ml,The detection limits were 0.06,2.5×10-3,2.8×10-3 ng,the quantitation limits were 0.19,8.2×10-3,9.2×10-3 ng,the average recoveries of three chemicals was 95.7%~103.7%. Conclution The method was specific,sensitive,easily and quickly,which could be used to detect Diazepam,Clonazepan and Estazolam in tranquilizing mind traditional Chinese medicine.

CHEN Rui, CHU Zhong-ying, ZHANG Ji-chun, TIAN Lan. Determination of forbidden addition drugs of Diazepam,Clonazepam and Estazolam in tranquilizing mind traditional Chinese medicine by UPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 457-459.
Citation: CHEN Rui, CHU Zhong-ying, ZHANG Ji-chun, TIAN Lan. Determination of forbidden addition drugs of Diazepam,Clonazepam and Estazolam in tranquilizing mind traditional Chinese medicine by UPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 457-459.
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