Determination of forbidden addition drugs of Diazepam,Clonazepam and Estazolam in tranquilizing mind traditional Chinese medicine by UPLC-MS/MS
- Received Date: 2011-05-29
- Rev Recd Date: 2011-06-15
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Key words:
- UPLC-MS/MS /
- traditional Chinese medicine /
- tranquilizing mind /
- addition drugs
Abstract: Objective To establish a specific method for determination of forbidden addition drugs of Diazepam,Clonazepan and Estazolam in tranquilizing mind Traditional Chinese Medicine. Methods Diazepam, Clonazepam and Estazolam were determined and analyzed by UPLC-MS/MS. ACQUITY BEH C18 analysis column(2.1 mm×50 mm,1.7 μm) was used with the mobile phase of 10 mmol/L ammonium acetate and acetonitrile(90:10) with gradient elution. The flow rate was 0.4 ml/min,and the detection wavelength was 254 nm.EIS ion source was used with positive ion monitoring. Results The standard curves of Diazepam,Clonazepam and Estazolam showed good linearity over the concentration rang of 109.8~878.4, 102.1~816.8,106.9~855.2 ng/ml,The detection limits were 0.06,2.5×10-3,2.8×10-3 ng,the quantitation limits were 0.19,8.2×10-3,9.2×10-3 ng,the average recoveries of three chemicals was 95.7%~103.7%. Conclution The method was specific,sensitive,easily and quickly,which could be used to detect Diazepam,Clonazepan and Estazolam in tranquilizing mind traditional Chinese medicine.
Citation: | CHEN Rui, CHU Zhong-ying, ZHANG Ji-chun, TIAN Lan. Determination of forbidden addition drugs of Diazepam,Clonazepam and Estazolam in tranquilizing mind traditional Chinese medicine by UPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 457-459. |