Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 29 Issue 6
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2011  >  29(6): 460-461

FEI Yang, WANG Hui, LING Shui-hua, ZHANG Guo-qing, ZHANG Zu-yan. Determination of chlorogenic acid content in wujin capsule by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 460-461.
Citation: FEI Yang, WANG Hui, LING Shui-hua, ZHANG Guo-qing, ZHANG Zu-yan. Determination of chlorogenic acid content in wujin capsule by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 460-461.
shu

Determination of chlorogenic acid content in wujin capsule by RP-HPLC

  • 1.

    Department of Pharmacy, Eastern Hepatobilliary Hospital, Second Military Medical University, Shanghai 200438, China

  • 2.

    Shanghai Hufeng biology technology Co, Ltd, Shanghai 200433, China

  • Received Date: 2011-06-27
  • Rev Recd Date: 2011-10-20
  • Objective To establish a RP-HPLC method for determination of chlorogenic acid in wujing capsule. Methods Agilent extend-C18 (4.6 mm×250 mm,5 μm)column was used. The mobile phase was acetonitrile: 0.1%formic acid (8：92). The flow rate was 1.0 ml/min, and the temperature was at 35 ℃. The detection wavelength was 327 nm. Determination of chlorogenic acid in wujin capsule of 5 batches of preparations. Results The calibration curve was at the range of 2.04~102 μg/ml(r=0.999 9), and the average recovery was 104.68 %,and RSD was 1.43%(n=6).5 batches of chlorogenic acid contents in wujin capsule were between 55.08~83.31 μg/g. Conclusion The method was sensitive and accurate, and could be used for the quality control of wujin capsule.
  • [1] 张家俊,胡德禹,宋宝安,等.乌骨藤化学成分和生物活性研究进展[J].贵州大学学报,2007,3(24): 213.
    [2] 于文静,王焕群.HPLC法测定消癌平口服液中绿原酸的含量[J].中国药品标准,2006,3(7):50.
    [3] 王月敏,夏素霞,于绍军,等.乌骨藤药材中绿原酸的含量测定方法研究[J].中国实验方剂学杂志,2007,7(13):12.
    [4] 赵陆华,相秉仁,谭喜莹.高效毛细管电泳法测定乌骨藤药材及其制剂中绿原酸的含量[J].中国现代应用药学杂志,2008,4(25):152.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(2694) PDF downloads(199) Cited by()

Related
Proportional views

Determination of chlorogenic acid content in wujin capsule by RP-HPLC

  • 1. Department of Pharmacy, Eastern Hepatobilliary Hospital, Second Military Medical University, Shanghai 200438, China
  • 2. Shanghai Hufeng biology technology Co, Ltd, Shanghai 200433, China
  • Received Date: 2011-06-27
  • Rev Recd Date: 2011-10-20

Abstract: Objective To establish a RP-HPLC method for determination of chlorogenic acid in wujing capsule. Methods Agilent extend-C18 (4.6 mm×250 mm,5 μm)column was used. The mobile phase was acetonitrile: 0.1%formic acid (8：92). The flow rate was 1.0 ml/min, and the temperature was at 35 ℃. The detection wavelength was 327 nm. Determination of chlorogenic acid in wujin capsule of 5 batches of preparations. Results The calibration curve was at the range of 2.04~102 μg/ml(r=0.999 9), and the average recovery was 104.68 %,and RSD was 1.43%(n=6).5 batches of chlorogenic acid contents in wujin capsule were between 55.08~83.31 μg/g. Conclusion The method was sensitive and accurate, and could be used for the quality control of wujin capsule.

FEI Yang, WANG Hui, LING Shui-hua, ZHANG Guo-qing, ZHANG Zu-yan. Determination of chlorogenic acid content in wujin capsule by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 460-461.
Citation: FEI Yang, WANG Hui, LING Shui-hua, ZHANG Guo-qing, ZHANG Zu-yan. Determination of chlorogenic acid content in wujin capsule by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 460-461.
shu

    HTML

Reference (4)

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return