TLC-UV测定水飞蓟宾单磷酸酯钠的含量
Determination of sodium salt of 11-phosphate by TLC-UV spectrophotometry
-
摘要: 目的 为了准确测定水飞蓟宾单磷酸酯钠的含量。方法 采用薄层层析对水飞蓟宾和水飞蓟宾单磷酸酯钠进行分离,用紫外分光光度法测定水飞蓟宾单磷酸酯钠的含量,测定波长 288nm。结果 线性范围 5～30μg/mL,相关系数为 0.9996,平均回收率为 98.93%,<>iRSD为 1.12%。结论 本法能有效消除水飞蓟宾对测定的干扰,准确测定水飞蓟宾单磷酸酯钠的真实含量。
-
关键词:
- 水飞蓟宾 /
- 水飞蓟宾单磷酸酯钠 /
- 薄层色谱-紫外分光光度法 /
- 含量测定
Abstract: Objective To determine the content of sodium salt of 11-phosphate. Methods Silybin and sodium salt of 11-phosphate were separated by TLC. The content of sodium salt of 11-phosphate in solution was determined by UV spectrophotometry. The detection wavelength was 288nm. Results The linear rang was 5～30μg/mL(r=0.9996).The recovery was 98.93%(n=5),RSD was 1.12%. Conclusion The method can eliminate the interference of silybin, the result was accurate.-
Key words:
- silybin /
- sodium salt of 11-phosphate /
- TCL-UV spectrophotometry /
- content determination
-
点击查看大图
计量
- 文章访问数: 2093
- HTML全文浏览量: 119
- PDF下载量: 1240
- 被引次数: 0
下载:
