HPLC法测定复方草玉梅含片中甘草酸的含量
Determination of the glycyrrhizic acid in compound anemone rivularis buccal tablets by HPLC
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摘要: 目的 建立复方草玉梅含片的含量测定方法。方法 采用高效液相色谱法,反相C18柱,以甲醇-0.2mol/L醋酸铵溶液-冰醋酸(53:47:1)为流动相,流速:1.0mL/min;检测波长250nm。结果 甘草酸单铵盐在0.0520. 468mg/mL范围内具有良好的线性关系,r=0.9996,精密度及回收率结果良好。结论 本法简单,方便,线性关系和重现性好,可有效地对本品进行质量控制。Abstract: Objective To establish a method to determine the Glycyrrhizic acid in Compound Anemone rivularis tablets. Methods Using HPLC with C18 column and mobile phase of methanol-0.2mol/L ammonium acetate solution-glacial acetic acid(53:47:1) with flow rate of 1.0 mL/min, the detection wavelength was 250nm. Result The linear range was 0.0520. 468mg/mL for ammonium glycyrrhizinate (r=0.9996), good linearity, precision and recovery were obtained. Conclusion The method is simple, rapid and accurate and could be used for quality control of compound anemone rivularis tablets.
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Key words:
- compound anemone rivularis tablets /
- glycyrrhizic acid /
- HPLC
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