刺五加总皂苷对大鼠心肌缺血性再灌注的保护作用及机制研究
Effects and mechanism of Acanthopanax senticosus saponins on myocardial ischemia and reperfusion in rats
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摘要: 目的: 观察刺五加总皂苷对大鼠心肌缺血性心功能损害的作用及其可能机制。 方法: 用麻醉大鼠心脏心肌缺血30 min、再灌注30 min造成离体心肌损伤模型,观察基础及再灌注后心功能及冠脉流量变化。 结果: 刺五加总皂苷大剂量组(20 mg/L)可改善大鼠心功能,中剂量及大剂量刺五加总皂苷(10及20 mg/L)可显著增加离体大鼠心脏基础冠脉流量,尚可显著增加缺血再灌注后心肌冠脉流量,增加冠脉流量的作用随给药浓度增加而加强。其对冠脉流量的作用可以被一氧化氮合酶抑制剂取消。 结论: 刺五加总皂苷对离体大鼠心脏基础心功能的作用较弱,但能显著改善心肌缺血再灌注后的心功能,这种心功能的增强机制可能主要是通过改善心肌缺血和通过增加一氧化氮扩张冠脉实现的。Abstract: Objective: To observe the protective effect of acanthopanax senticosus saponins(ASS) on myocardial ischemia-reperfusion in rats and to find a possible mechanism. Methods: An in vitro myocardial injury model was made by 30 min of ischemia followed by 30 min of reperfusion in the isolated rat heart from anesthetized rat.The change of heart function and coronary flow in normal and reperfusion conditions were observed. Results: ASS can improve the heart function at the dose of 20 mg/L;and at the dose of 10 mg/L and 20 mg/L ASS it can increase the coronary flow in normal and reperfusion conditions at dose-dependent ways;The effects can be cancelled by nitric oxide syntheses inhibitor. Conclusion: ASS has a weak influence on the normal heart function in vitro rats heart,but significantly improve the heart function after myocardial ischemia and reperfusion in rats.This mechanism may be related to the improvement in the myocardial ischemia and the coronary artery through increasing the nitric oxide.
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