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作为基因输送载体的壳聚糖衍生物研究进展

李晏

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文章导航 > 药学实践与服务  > 2011 >  29(1): 8-10,61
李晏. 作为基因输送载体的壳聚糖衍生物研究进展[J]. 药学实践与服务, 2011, 29(1): 8-10,61.
引用本文: 李晏. 作为基因输送载体的壳聚糖衍生物研究进展[J]. 药学实践与服务, 2011, 29(1): 8-10,61.
shu
LI Yan. Research progress of chitosan derivatives as gene delivery vector[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 8-10,61.
Citation: LI Yan. Research progress of chitosan derivatives as gene delivery vector[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 8-10,61.
shu

作为基因输送载体的壳聚糖衍生物研究进展

  • 解放军第411医院,上海 200081

Research progress of chitosan derivatives as gene delivery vector

  • Department of Pharmacy, 411th Hospital of PLA, Shanghai 200081,China

  • 摘要: 壳聚糖作为基因载体,目前存在的主要问题是还不能达到足够高的表达效率。其中主要原因是壳聚糖在pH 7.4的生理环境下溶解度较差,壳聚糖与DNA形成的复合物在生理环境下的稳定性较差,缺乏细胞靶向性。本文综述了作为基因输送载体的壳聚糖衍生物研究进展,为进一步研究和开发壳聚糖衍生物提供依据和参考。
    Abstract: Despite the advantages of chitosan as a non-viral gene delivery vector, the application of this system is significantly limited by its poor solubility (the amino groups on chitosan are only partially protonated at physiological pH 7.4), poor stability of the polyplex at physiological pH, low cell specificity and therefore low transfection efficiency. Chitosan structure modification or additive incorporation is an effective way to improve the stability of the polyplex in biological fluids, enhance targeted cell delivery and facilitate endo-lysosomal release of the complex. In this paper, chitosan derivatives as gene delivery vector were reviewed to facilitate the process of chitosan vector development for clinical application.
  • [1] Brus C, Petersen H, Aigner A, et al. Efficiency of polyethylenimines and polyethyleniminegraft-poly (ethylene glycol) block copolymers to protect oligonucleotides against enzymatic degradation[J]. Eur.J Pharm Biopharm, 2004, (57): 427.
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    [4] Kunath K, von Harpe A, Petersen H, et al. The structure of PEG-modified poly(ethylene imines) influences biodistribution and pharmacokinetics of their complexes with NF-kappaB decoy in mice[J].Pharm Res,2002, (19) : 810.
    [5] Zhang Y, Chen J, Zhang Y, et al. A novel PEGylation of chitosan nanoparticles for gene delivery[J].Biotechnol Appl Biochem, 2007 ,(46) :197.
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    [19] Satoh T, Kano H, Nakatani M, et al. 6-Amino-6-deoxy chitosan. Sequential chemical modifications at the C-6 positions of N-phthaloylchitosan and evaluation as a gene carrier[J].Carbohydr Res,. 2006, (341) : 2406.
    [20] Park IK, Ihm JE, Park YH, et al. Galactosylated chitosan(GC)-graftpoly(vinyl pyrrolidone) (PVP) as hepatocytetargeting DNA carrier: preparation and physicochemical characterization of GCgraft-PVP/DNA complex (1) [J]. J Control Release,2003, (86) : 349.
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  • 收稿日期:  2010-09-20
  • 修回日期:  2010-10-21

作为基因输送载体的壳聚糖衍生物研究进展

  • 解放军第411医院,上海 200081
  • 收稿日期: 2010-09-20
  • 修回日期: 2010-10-21

摘要: 壳聚糖作为基因载体,目前存在的主要问题是还不能达到足够高的表达效率。其中主要原因是壳聚糖在pH 7.4的生理环境下溶解度较差,壳聚糖与DNA形成的复合物在生理环境下的稳定性较差,缺乏细胞靶向性。本文综述了作为基因输送载体的壳聚糖衍生物研究进展,为进一步研究和开发壳聚糖衍生物提供依据和参考。

English Abstract

李晏. 作为基因输送载体的壳聚糖衍生物研究进展[J]. 药学实践与服务, 2011, 29(1): 8-10,61.
引用本文: 李晏. 作为基因输送载体的壳聚糖衍生物研究进展[J]. 药学实践与服务, 2011, 29(1): 8-10,61.
shu
LI Yan. Research progress of chitosan derivatives as gene delivery vector[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 8-10,61.
Citation: LI Yan. Research progress of chitosan derivatives as gene delivery vector[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 8-10,61.
shu

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