RP-HPLC法测定中药木贼中黄酮醇苷的含量
Determination of a flavonol glycoside in Equisetum hiemale by RP-HPLC
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摘要: 目的 建立以高效液相法测定中药木贼中一个黄酮醇苷山奈酚-3-双葡萄糖苷的含量方法。 方法 色谱柱为Calesil ODS-100(250 mm×4.6 mm, 5 μm),流动相为甲醇-水(45:55),流速为1.0 ml/min, 检测波长254 nm, 柱温30 ℃,进样量10 μl。 结果 山奈酚-3,7-双葡萄糖苷的检测浓度在0.003 12~0.104 mg/ml范围内与峰积分面积呈良好线性关系(r=0.999 9), 平均回收率为100.2%,RSD=1.74% (n=9)。3个不同产地批号的样品中山奈酚-3-双葡萄糖苷的含量均在0.30%以上。 结论 该方法简便、快捷、重复性好,可作为木贼药材的质量控制的方法。
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关键词:
- 木贼 /
- 黄酮醇苷 /
- 山奈酚-3-双葡萄糖苷 /
- 反相高效液相法 /
- 含量测定
Abstract: Objective To establish a RP-HPLC method for determination of a flavonol glycoside in Equisetum hiemale. Methods The separation was performed on Calesil ODS-100(250 mm×4.6 mm,5 μm). Mobile phase consisted of methanol-water (45:55) with flow rate of 1.0 ml/min and detection wavelength of 254 nm. The column temperature was 30 ℃ and injection volume was 10 μl. Results The linear range of the flavonol glycoside was 0.003 12~0.104 mg/ml with average recovery of 100.2% ( RSD=1.74%, n=9 ). The method was applied to samples of Equisetum hiemale from different locations. Conclusion The established method was proved to be simple, reproducible, accurate and could be used to evaluate the quality of Equisetum hiemale.-
Key words:
- Equisetum hiemale /
- flavonal glycoside /
- kaempferol-3-diglucoside /
- RP-HPLC /
- Content determination
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[1] 中国药典2010年版[S]. 一部.2010:58. [2] 李淑惠,靳丹虹,李德坤,等. 木贼科植物研究概况I.化学成分研究[J].中草药.2000,31(7):附12. [3] 张承忠,赵 磊,李 冲,等. 木贼化学成分研究[J].中草药.2002,33(11):978. [4] 朴惠顺,金光洙. 木贼的化学成分和药理作用研究进展[J].时珍国医国药,2006,17(6):1077. [5] 高汉成,何明三,韩宁涛. 木贼乙醇提取物中槲皮素HPLC法含量测定的研究[J].湖北中医学院学报,1999,1(3):29. [6] 张 宪,赵惠萍,张小茜,等. 木贼质量标准的研究[J].中国中医药信息杂志,2005,12(5):48. [7] 赵 磊,李 冲,张承忠,等. 木贼中三种黄酮苷的含量测定[J].中国现代应用药学杂志,2005,22(2):159. [8] 隋长惠,王玉洁,王昌库.木贼中山奈酚的含量测定[J].沈阳药科大学学报,1996,13(2):121. [9] Do Monte FH, dos Santos JG Jr, Russi M, et al. Antinociceptive and anti-inflammatory properties of the hydroalcoholic extract of stems from Equisetum arvense L. in mice[J]. Pharmacological research, 2004(49):239. [10] Correia H, Gonzalez-Paramas A, Amaral M.T., et al. Characterisation of polyphenols by HPLC-PAD-ESI/MS and antioxidant activity in Equisetum telmateia[J]. Phytochemical analysis, 2005(16):380. -
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