HPLC-TOFMS法快速分离与鉴别丹香冠心注射液中的化学成分
Rapid separation and identification of chemical components in Danxiangguanxin injection by HPLC-TOFMS
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摘要: 目的 采用HPLC-TOFMS对丹香冠心注射液中化学成分进行快速分离与鉴别。 方法 色谱柱Agilent Zorbax SB-C18柱(3.0 mm × 100 mm, 3.5 μm),以0.5 %醋酸(A)-乙腈(B)为流动相,梯度洗脱(v/v),0~25 min, 2%~33% B; 25~30 min, 33%~95% B,进样量5 μl,流速0.5 ml/min,柱温为30 ℃,飞行时间质谱配有ESI离子源,质量数扫描范围m/z 100~1 000。 结果 采用HPLC-TOFMS方法对丹香冠心注射液制液进行了在线化学成分分离,共鉴别出丹香冠心注射液中31个化学成分。 结论 采用HPLC-TOFMS方法对丹香冠心注射液的化学成分进行快速分析鉴别,可为中药丹香冠心注射液的药效物质基础和质量控制研究奠定基础。
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关键词:
- 丹香冠心注射液 /
- HPLC-TOFMS /
- 鉴别
Abstract: Objective To separate and identify the components in Danxiangguanxin injection by high performance liquid chromatography time-of-flight mass spectrometry (HPLC-TOFMS). Methods Agilent Zorbax SB-Cl8 column (3.0 mm×100 mm, 3.5 μm) was used for separation and identification of chemical components in Danxiangguanxin injection with a mobile phase of 0.5 % acetic acid (A) and acetonitrile (B) in gradient elution. 0~25 min, 2%~33% B; 25~30 min, 33%~95% B; the flowing rate was 0.5 ml/min and the injection volume was 5 μl. And the time-of-flight mass spectrometer was equipped with an EIS ion source. Scanning mass range was ranged between m/z 100~1000. Results Thirty-one chemical compounds in Danxiangguanxin injection were identified unequivocally. Conclusion Chromatographic demonstration of chemical compounds in Danxiangguanxin injection could provide foundation for the further metabolism and action mechanism study of Danxiangguanxin injection.-
Key words:
- Danxiangguanxin injection /
- HPLC-TOFMS /
- identification
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[1] 中国药典2010版.一部[S].2010: 70, 213. [2] 李小娟,沃兴德.丹参酮的药理作用研究进展[J].现代生物医学进展,2008,8(12):2378. [3] 黄赵刚,刘 钢,夏 泉,等. HPLC法测定不同厂家丹参注射液中丹参素和原儿茶醛的含量[J].安徽医药,2008,12(11):1055. [4] 朱 庆,谢松强,肖晋芳,等.复方降香胶囊的抗心肌缺血作用[J].沈阳药科大学学报,2006,23(9):586. [5] 王玉团,彭艳丽,李 勇. HPLC法测定注射用丹香冠心中丹参素钠和原儿茶醛的含量[J].食品与药品, 2007,9(5):21. [6] 孙国祥,孙金山,赵 新. 丹参水溶性成分HPLC数字化指纹图谱研究[J].中国南药,2008,6(3):356. [7] Zeng GF, Xiao HB, Liu JX, et al. Identification of phenolic constituents in Radix Salvia miltiorrhizae by liquid chromatography/electrospray ionization mass spectrometry[J]. Rapid Communications in Mass Spectrometry,2006,20:499. [8] Yang M, Liu AH, Guan SH, et al. Characterization of tanshinones in the roots of Salvia Miltiorrhiza (Dan-shen) by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry[J]. Rapid Communications in Mass Spectrometry,2006,20:1268. [9] 王 靖,张铁军,廖茂梁. HPLC法测定降香挥发油羟丙基-[3-环糊精包合物中反式一橙花叔醇]J].天津中医药大学学报,2006,25(2):92. [10] Tao Y, Wang Y. Bioactive sesquiterpenes isolated from the essential oil of Dalbergia odorifera T. Chen[J]. Fitoterapia 2010, 81:393. -
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