用细胞膜色谱法和HPLC-TOF/MS研究附子中的有效成分
Study on effective components from roots of Aconitum carmichaeli through cell membrane chromatography and HPLC-TOF/MS method
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摘要: 目的 通过细胞膜色谱技术和高效液相-飞行时间质谱(HPLC-TOF/MS)技术相结合,初步筛选和鉴定附子中的有效成分。 方法 初步筛选采用自制的大鼠心肌细胞膜色谱柱,流动相为10 mmol/L 磷酸盐缓冲溶液,流速为0.2 ml/min。色谱分离采用SHISEIDO CAPCELL PAK C18柱,流动相为0.1％甲酸水溶液和乙腈,梯度洗脱,流速为1.0 ml/min。质谱鉴定采用TOF/MS,正离子模式扫描。 结果 确定了附子中8个可能的有效成分。 结论 通过细胞膜色谱技术和HPLC-TOF/MS技术相结合,为从天然产物中快速寻找新的活性成分提供了途径。
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关键词:
- 细胞膜色谱 /
- 高效液相-飞行时间质谱 /
- 附子
Abstract: Objective To screen the effective components from roots of Aconitum carmichaeli by a cell membrane chromatography (CMC) combining with a HPLC-TOF/MS method. Methods Screening was conducted on a rat cardiac muscle CMC column, and mobile phase was 10 mmol/L phosphate buffer with a flow rate of 0.2 ml/min. Separation was conducted on a SHISEIDO CAPCELL PAK C18 column. The mobile phase consisted of 0.1% formic acid and acetonitrile using gradient elution with a flow rate of 1.0 ml/min. Identification was conducted by using TOF/MS in positive ion mode. Results Eight potential effective constituents were determined. Conclusion The combination of CMC and HPLC-TOF/MS method had provided a new way to discover active components targeting membrane receptors from natural products.-
Key words:
- cell membrane chromatography /
- HPLC-TOF/MS /
- Aconitum carmichaeli
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