基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究

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基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究

范加腾, 陆峰, 许激扬

文章导航 > 药学实践与服务 > 2019 > 37(2): 156-161,169

范加腾, 陆峰, 许激扬. 基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究[J]. 药学实践与服务, 2019, 37(2): 156-161,169. doi: 10.3969/j.issn.1006-0111.2019.02.011

引用本文:

范加腾, 陆峰, 许激扬. 基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究[J]. 药学实践与服务, 2019, 37(2): 156-161,169. doi: 10.3969/j.issn.1006-0111.2019.02.011

FAN Jiateng, LU Feng, XU Jiyang. Research on the inhibitory effect of amphotericin B against Candida albicans by D-SERS[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 156-161,169. doi: 10.3969/j.issn.1006-0111.2019.02.011

Citation:

FAN Jiateng, LU Feng, XU Jiyang. Research on the inhibitory effect of amphotericin B against Candida albicans by D-SERS[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 156-161,169. doi: 10.3969/j.issn.1006-0111.2019.02.011

基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究

doi: 10.3969/j.issn.1006-0111.2019.02.011

中国药科大学生命科学与技术学院, 江苏南京, 210000

Research on the inhibitory effect of amphotericin B against Candida albicans by D-SERS

College of life science and technology, China Pharmaceutical University, Nanjing 210000, China

摘要: 目的建立动态表面增强拉曼光谱法（D-SERS）快速表征两性霉素B （AMB）对白色念珠菌的抑制作用。方法采用D-SERS法，选用敏感的白色念珠菌分别与不同浓度的AMB相互作用，在不同的作用时间下，通过考察拉曼位移在656 cm^-1和729 cm^-1处的峰强比和药物浓度之间的关系，分析AMB对白色念珠菌拉曼光谱的影响。结果在一定药物浓度和作用时间下，峰强比与AMB浓度呈负相关，可用于描述AMB对白色念珠菌的抑制作用，且药物浓度低于其最小抑菌浓度。结论该方法简便、快捷，实现了AMB对白色念珠菌的快速表征，同时为表征更多药物-病原体相互作用提供了更简单、快速的检测方法。
- 白色念珠菌 /
- 两性霉素B /
- 动态表面增强拉曼光谱法
Abstract: Objective To develop a rapid method to characterize the inhibitory effect of amphotericin B(AMB) against Candida albicans by dynamic surface enhanced Raman spectroscopy(D-SERS). Methods D-SERS were applied to analyze the effect of AMB on the Raman spectra of C.albicans by examining the relationship between the peak intensity ratio and the concentration of drug at Raman shift at 656 cm^-1 and 729 cm^-1 under different interaction time by a sensitive C.albicans and different AMB concentrations. Results The peak intensity ratio was negatively correlated with the concentration of AMB,which could be used to describe the inhibitory effect of AMB on C.albicans in a certain concentration and action time. Conclusion This method could characterize the inhibitory effect of AMB against C.albicans rapidly,which provided a simpler and faster detection method for characterizing more drug-pathogen interactions.
- Candida albicans /
- amphotericin B /
- dynamic surface enhanced Raman spectroscopy

[1]	廖万清.深部真菌感染治疗进展[C].2003中国中西医结合皮肤性病学术会议论文汇编,2003,597-600.
[2]	PFALLER M A. Nosocomial candidiasis:emerging species,reservoirs,and modes of transmission[J]. Clinical Infectious Diseases,1996,22(2):S89-S94.
[3]	SZWEDA P,GUCWA K,ROMANOWSKA E,et al. Mechanisms of azole resistance among clinical isolates of Candida glabrata in Poland[J]. J Med Microbiol,2015,64(6):610-619.
[4]	WHITE T C,MARR K A,BOWDEN R A. Clinical,cellular,and molecular factors that contribute to antifungal drug resistance[J]. Clin Microbiol Rev,1998,11(2):382-402.
[5]	FEKETE-FORGÁCS K,GYÜRE L,LENKEY B. Changes of virulence factors accompanying the phenomenon of induced fluconazole resistance in Candida albicans[J]. Mycoses,2000,43(7-8):273-279.
[6]	SANGUINETTI M,POSTERARO P,POSTERARO B. Echinocandin antifungal drug resistance in Candida species:a cause for concern[J]. Curr Infect Dis Rep,2010,12(6):437-443.
[7]	KHAN A A,JABEEN M,ALANAZI A M,et al. Antifungal efficacy of amphotericin B encapsulated fibrin microsphere for treating Cryptococcus neoformans infection in Swiss albino mice[J]. Braz J Infect Dis,2016,20(4):342-348.
[8]	BREHM-STECHER B F,JOHNSON E A. Single-cell microbiology:tools,technologies,and applications[J]. Microbiol Mol Biol Rev,2004,68(3):538-559,table of contents.
[9]	JORGENSEN J,FERRARO M. Antimicrobial susceptibility testing:a review of general principles and contemporary practices[J]. Clin Infect Dis,2009,49(11):1749-1755.
[10]	LI P,LIU H,YANG L,et al. The time-resolved D-SERS vibrational spectra of pesticide thiram[J]. Talanta,2013,117(22):39-44.
[11]	HE W,ZHOU Y T,WAMER W G,et al. Mechanisms of the pH dependent generation of hydroxyl radicals and oxygen induced by Ag nanoparticles[J]. Biomaterials,2012,33(30):7547-7555.
[12]	LIU H,YANG Z,MENG L,et al. Three-dimensional and time-ordered surface-enhanced raman scattering hotspot matrix[J]. J Am Chem Soc,2014,136(14):5332-5341.
[13]	ZHU Y,LI M,YU D,et al. A novel paper rag as ‘D-SERS’ substrate for detection of pesticide residues at various peels[J]. Talanta,2014,128:117-124.
[14]	YANG L,LI P,LIU H,et al. A dynamic surface enhanced Raman spectroscopy method for ultra-sensitive detection:from the wet state to the dry state[J]. Chem Soc Rev,2015,44(10):2837-2848.
[15]	TANG X,CAI W,YANG L,et al. Monitoring plasmon-driven surface catalyzed reactions in situ using time-dependent surface-enhanced Raman spectroscopy on single particles of hierarchical peony-like silver microflowers[J]. Nanoscale,2014,6(15):8612-8616.
[16]	WU M,LI H,LV D,et al. Dynamic-SERS spectroscopy for the in situ discrimination of xanthine analogues in ternary mixture[J]. Anal Bioanal Chem,2017,409(23):5569-5579.
[17]	FANG F,QI Y,LU F,et al. Highly sensitive on-site detection of drugs adulterated in botanical dietary supplements using thin layer chromatography combined with dynamic surface enhanced Raman spectroscopy[J]. Talanta,2016,146:351-357.
[18]	LEE P C,MEISEL D. Adsorption and surface-enhanced Raman of dyes on silver and gold sols[J]. J Phys Chem,1982,86(17):3391-3395.
[19]	JANA N R,GEARHEART L,MURPHY C J. Seed-mediated growth approach for shape-controlled synthesis of spheroidal and rod-like gold nanoparticles using a surfactant template[J].Adv Mater,2001,13(18):1389-1393.
[20]	PREMASIRI W R,LEE J C,SAUER-BUDGE A,et al.The biochemical origins of the surface-enhanced Raman spectra of bacteria:a metabolomics profiling by SERS[J]. Anal Bioanal Chem,2016,408(17):4631-4647.
[21]	SAYIN I,KAHRAMAN M,SAHIN F,et al.Characterization of yeast species using surface-enhanced Raman scattering[J]. Appl Spectrosc,2009,63(11):1276-1282.

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基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究

doi: 10.3969/j.issn.1006-0111.2019.02.011

中国药科大学生命科学与技术学院, 江苏南京, 210000

收稿日期: 2018-05-05
修回日期: 2018-11-13

关键词:

白色念珠菌 /

两性霉素B /

动态表面增强拉曼光谱法

摘要: 目的建立动态表面增强拉曼光谱法（D-SERS）快速表征两性霉素B （AMB）对白色念珠菌的抑制作用。方法采用D-SERS法，选用敏感的白色念珠菌分别与不同浓度的AMB相互作用，在不同的作用时间下，通过考察拉曼位移在656 cm^-1和729 cm^-1处的峰强比和药物浓度之间的关系，分析AMB对白色念珠菌拉曼光谱的影响。结果在一定药物浓度和作用时间下，峰强比与AMB浓度呈负相关，可用于描述AMB对白色念珠菌的抑制作用，且药物浓度低于其最小抑菌浓度。结论该方法简便、快捷，实现了AMB对白色念珠菌的快速表征，同时为表征更多药物-病原体相互作用提供了更简单、快速的检测方法。

English Abstract

范加腾, 陆峰, 许激扬. 基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究[J]. 药学实践与服务, 2019, 37(2): 156-161,169. doi: 10.3969/j.issn.1006-0111.2019.02.011

引用本文:

范加腾, 陆峰, 许激扬. 基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究[J]. 药学实践与服务, 2019, 37(2): 156-161,169. doi: 10.3969/j.issn.1006-0111.2019.02.011

FAN Jiateng, LU Feng, XU Jiyang. Research on the inhibitory effect of amphotericin B against Candida albicans by D-SERS[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 156-161,169. doi: 10.3969/j.issn.1006-0111.2019.02.011

Citation:

FAN Jiateng, LU Feng, XU Jiyang. Research on the inhibitory effect of amphotericin B against Candida albicans by D-SERS[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 156-161,169. doi: 10.3969/j.issn.1006-0111.2019.02.011

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