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五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用

陈迁,  陈孟莉,  叶兆波

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文章导航 > 药学实践与服务  > 2012 >  30(1): 25-28,31
陈迁, 陈孟莉, 叶兆波. 五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用[J]. 药学实践与服务, 2012, 30(1): 25-28,31. doi: 10.3969/j.issn.1006-0111.2012.01.006
引用本文: 陈迁, 陈孟莉, 叶兆波. 五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用[J]. 药学实践与服务, 2012, 30(1): 25-28,31. doi: 10.3969/j.issn.1006-0111.2012.01.006
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CHEN Qian, CHEN Meng-li, YE Zhao-bo. Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 25-28,31. doi: 10.3969/j.issn.1006-0111.2012.01.006
Citation: CHEN Qian, CHEN Meng-li, YE Zhao-bo. Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 25-28,31. doi: 10.3969/j.issn.1006-0111.2012.01.006
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五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用

doi: 10.3969/j.issn.1006-0111.2012.01.006
  • 1.

    中国人民解放军总医院临床药理研究室,北京 100853

  • 2.

    香港中文大学中医学院,香港

基金项目: 国家自然科学基金资助项目(81041030).

Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat

  • 1.

    Department of clinical pharmacology research, General Hospital of PLA, Beijing 100853 China

  • 2.

    College of traditional Chinese medicine , The Chinese University of Hong Kong , Hong Kong,China

  • 摘要: 目的 在前期研究工作的基础上,对具有肝脏保护作用的中药五子衍宗复方(WZ)进行进一步的分离,考察WZ复方提取物中总多糖(TPC)对慢性酒精中毒大鼠肝脏的保护作用并探讨其可能的作用机制。 方法 本实验采用改良的Lieber-DeCarli液体酒精饲料喂养致慢性酒精性肝损伤大鼠模型,应用TPC治疗,观察血清生化指标和病理学改变,考察对肝脏氧化应激、脂质过氧化指标和细胞因子TNF-α水平及TNF-α mRNA 表达影响。 结果 TPC能够显著降低升高的肝脏指数和血清ALT、AST水平,减轻肝脏组织病理学改变;减
    Abstract: Objective To investigate the protect effect and mechanism of total polysaccharides (TPC) isolated from 50% ethanol extract of Wuzi-Yanzong pill (WZ) against chronic alcohol-induced injury in Rat . Methods The effect of TPC on chronic ethanol hepatotoxicity was investigated in modified Lieber-DeCarli rat model. Hepatocellular damage indicator (Serum ALT, AST) and histopathological examination of liver in rat were evaluated. MDA content, SOD activity and GSH content in liver were measured. TNF-α content and TNF-α mRNA expression were determined. Results TPC protected against chronic ethanol-induced hepatotoxicity in rat, as evidenced by decreased plasma transaminases activities and signally improve the liver pathological changes induced by alcohol. TPC significantly inhibited the increase of MDA content, improved SOD activity and prevented GSH depletion. TPC significantly alleviated hepatic TNF-α production and decreased the expression of TNF-α mRNA. Conclusion TPC possessed potent hepatoprotective activities in chronic alcohol-induced injury rat model. The suppressive effect of TPC on chronic alcohol induced liver injury might be attributed to the antioxidant action and suppress TNF-α expression at protein and gene level.
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    [2] 全国酒精性肝病调查协作组. 全国酒精性肝病的多中心调查分析[J]. 中华消化杂志 2007; 27: 231.
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    [5] Chen ML, Ip SP, Tsai SH, et al. Biochemical mechanism of Wu-Zi-Yan-Zong-Wan, a traditional Chinese herbal formula, against alcohol-induced oxidative damage in CYP2E1 cDNA-transfected HepG2 (E47) cells[J]. J Ethnopharmacol, 2010, 128: 116.
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  • 收稿日期:  2011-08-11
  • 修回日期:  2011-10-14

五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用

doi: 10.3969/j.issn.1006-0111.2012.01.006
  • 1. 中国人民解放军总医院临床药理研究室,北京 100853
  • 2. 香港中文大学中医学院,香港
    • 基金项目:  国家自然科学基金资助项目(81041030).
  • 收稿日期: 2011-08-11
  • 修回日期: 2011-10-14

摘要: 目的 在前期研究工作的基础上,对具有肝脏保护作用的中药五子衍宗复方(WZ)进行进一步的分离,考察WZ复方提取物中总多糖(TPC)对慢性酒精中毒大鼠肝脏的保护作用并探讨其可能的作用机制。 方法 本实验采用改良的Lieber-DeCarli液体酒精饲料喂养致慢性酒精性肝损伤大鼠模型,应用TPC治疗,观察血清生化指标和病理学改变,考察对肝脏氧化应激、脂质过氧化指标和细胞因子TNF-α水平及TNF-α mRNA 表达影响。 结果 TPC能够显著降低升高的肝脏指数和血清ALT、AST水平,减轻肝脏组织病理学改变;减

English Abstract

陈迁, 陈孟莉, 叶兆波. 五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用[J]. 药学实践与服务, 2012, 30(1): 25-28,31. doi: 10.3969/j.issn.1006-0111.2012.01.006
引用本文: 陈迁, 陈孟莉, 叶兆波. 五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用[J]. 药学实践与服务, 2012, 30(1): 25-28,31. doi: 10.3969/j.issn.1006-0111.2012.01.006
shu
CHEN Qian, CHEN Meng-li, YE Zhao-bo. Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 25-28,31. doi: 10.3969/j.issn.1006-0111.2012.01.006
Citation: CHEN Qian, CHEN Meng-li, YE Zhao-bo. Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 25-28,31. doi: 10.3969/j.issn.1006-0111.2012.01.006
shu

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