Preparation of quercetin liposome by film-ultrasonic technique
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摘要: 目的 以粒径和包封率为指标,优选槲皮素脂质体的制备工艺。 方法 以氢化大豆磷脂(HSPC)、胆固醇(CH)为膜材,采用薄膜超声法制备脂质体。通过正交设计优化处方工艺,利用马尔文动态光散射粒径测定仪测定脂质体的粒径,鱼精蛋白沉淀法分离游离药物,HPLC法测定脂质体中槲皮素(QU)的包封率。 结果 最佳处方工艺为:HSPC:CH=3:1、HSPC:QU=20:1、探头超声(600 W)9 min。 结论 薄膜超声法适于实验室条件下制备槲皮素脂质体。Abstract: Objective To study the preparation method of quercetin liposome and screen the optimal technological conditions by the particle sizes and the encapsulation efficiencies of quercetin. Method Liposomes were made of hydrogenated soybean phosphatidylcholine(HSPC) and cholesterol(CH) by film evaporation and probe ultrasonic technique, then orthogonal design was adopted to screen the optimal conditions. The particle sizes were detected by Zetasizer Nano, while the encapsulation efficiency of quercetin (QU) were determined by HPLC after the free drug was separated by protamine sedimentation method. Results The optimal technological conditions of quercetin liposome were as follows: HSPC∶CH=3∶1, HSPC:QU=20:1, the time of probe ultrasonic (600 w) was 9 minutes. Conclusion Film evaporation and probe ultrasonic technique could be suitable for laboratory to prepare quercetin liposome.
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[1] 王 黎,侯宝光,侯新朴,等.氢化与非氢化卵磷脂对阿霉素脂质体体内外稳定性的影响[J].药学学报,2001,36(6):444. [2] 丁燕飞,姚 瑶,陶昱斐,等.槲皮素纳米脂质体的处方工艺优化[J].中草药,2008,39(4):522. [3] 孙维彤,黄桂华,叶杰胜,等.鱼精蛋白凝聚法测定脂质体和纳米脂质体包封率[J].中国药学杂志,2006,41(22):1717. -
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