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LC-MS/MS测定大鼠血浆中虎杖苷浓度

丁雪鹰,  侯晓丽,  孙铭学,  肖凯

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丁雪鹰, 侯晓丽, 孙铭学, 肖凯. LC-MS/MS测定大鼠血浆中虎杖苷浓度[J]. 药学实践与服务, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012
引用本文: 丁雪鹰, 侯晓丽, 孙铭学, 肖凯. LC-MS/MS测定大鼠血浆中虎杖苷浓度[J]. 药学实践与服务, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012
shu
DING Xue-ying, HOU Xiao-li, SUN Ming-xue, XIAO Kai. Determination of polydatin in rats plasma by LC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012
Citation: DING Xue-ying, HOU Xiao-li, SUN Ming-xue, XIAO Kai. Determination of polydatin in rats plasma by LC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012
shu

LC-MS/MS测定大鼠血浆中虎杖苷浓度

doi: 10.3969/j.issn.1006-0111.2012.01.012
  • 1.

    第二军医大学药学院药剂学教研室,上海 200433

  • 2.

    第二军医大学海医系毒物药物研究室,上海 200433

基金项目: 上海市科委中药现代化项目(08DZ1971504);国家自然科学基金(20872179,30472141).

Determination of polydatin in rats plasma by LC-MS/MS

  • 1.

    Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • 2.

    Laboratory of Toxicology & Pharmacology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China

  • 摘要: 目的 建立测定大鼠血浆中虎杖苷浓度的LC-MS/MS方法。 方法 采用LC-MS/MS测定大鼠血浆中虎杖苷的浓度,以二苯乙烯苷为内标,血浆样品用乙腈沉淀蛋白,色谱柱为Agilent Zorbax SB-C18 (100 mm×2.1 mm, 3.5 μm),流动相为甲醇-乙腈-0.1%甲酸水溶液(18∶15∶67),流速0.3 ml/min,柱温30 ℃。 结果 虎杖苷的线性范围为1.0~5 000.0 ng/ml (r=0.998 4),最低定量检测浓度为1.0
    Abstract: Objective To establish a LC-MS/MS method for determination of polydatin concentration in rats plasma. Methods The plasma concentration of polydatin was determined by LC-MS/MS. Using stilbene glucoside as internal standard, after precipitation of the plasma proteins with methanol, the analytes were separated on an agilent zorbax SB-C18 reversed-phase column with methanol-acetonitrile- 0.1% formic acid (18 : 15 : 67, v/v) and detected by electrospray ionization (ESI) mass spectrometry in negative multiple reaction monitoring (MRM) mode. The flow rate was 0.3 ml/min. Column temperature was maintained at 30 ℃. Results The calibration curves with good linearity (r=0.998 4 for plasma sample) were obtained in the range of 1.0~5000.0 ng/ml for polydatin. The lower limit of quantification (LLOQ) was 1.0 ng/ml. Recoveries were around 78% for the extraction from rats plasma, and good precision and accuracy were achieved. The intra-day and inter-day RSD were both less than 15.0%. This method was feasible for the evaluation of pharmacokinetic profiles of polydatin in rats. Conclusion This method was simple, selective and sensitive. It's suitable for the pharmacokinetic research of polydatin in rats plasma.
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    [2] 高守红,杨少麟,范国荣. 虎杖苷的研究进展[J].药学实践杂志,2005,23(3):145.
    [3] 李蓟龙,尹京湘,张 力,等. 虎杖中白藜芦醇对豚鼠左心室流出道自律组织电活动的影响[J]. 中国应用生理学杂志,2010,26(3):311,364.
    [4] 刘 铭,刘 华,张国平,等. 虎杖苷对脑出血性损伤大鼠血清白细胞介素-1β和脑组织Bcl-2蛋白表达的影响[J]. 中草药,2010, 41(12):2010.
    [5] Gao S, Fan G, Hong Z, et al. HPLC determination of polydatin in rat biological matrices: application to pharmacokinetic studies[J]. J Pharmaceut Biomed,2006, 41(1): 240.
    [6] 吕春艳,张兰桐,袁志芳,等. 虎杖苷在大鼠体内的药动学特点和组织分布研究[J].中草药,2006,38(2):235.
    [7] 林建海,李笑宏,唐跃年,等. 白藜芦醇苷的HPLC法分析及其在苗猪体内的药代动力学研究[J]. 药物分析杂志, 2001,21(05):325.
    [8] 杨润涛,周四元,章 翔,等.液相色谱-串联质谱法同时测定血浆中白藜芦醇苷及其代谢产物[J]. 分析化学,2007,35(9):1309.
    [9] Zhou S, Yang R,Teng Z,et al. Dose-dependent absorption and metabolism of trans-polydatin in rats[J]. J Agr Food Chem, 2009,57(11): 4572.
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出版历程
  • 收稿日期:  2011-02-10
  • 修回日期:  2011-12-21

LC-MS/MS测定大鼠血浆中虎杖苷浓度

doi: 10.3969/j.issn.1006-0111.2012.01.012
  • 1. 第二军医大学药学院药剂学教研室,上海 200433
  • 2. 第二军医大学海医系毒物药物研究室,上海 200433
    • 基金项目:  上海市科委中药现代化项目(08DZ1971504);国家自然科学基金(20872179,30472141).
  • 收稿日期: 2011-02-10
  • 修回日期: 2011-12-21

摘要: 目的 建立测定大鼠血浆中虎杖苷浓度的LC-MS/MS方法。 方法 采用LC-MS/MS测定大鼠血浆中虎杖苷的浓度,以二苯乙烯苷为内标,血浆样品用乙腈沉淀蛋白,色谱柱为Agilent Zorbax SB-C18 (100 mm×2.1 mm, 3.5 μm),流动相为甲醇-乙腈-0.1%甲酸水溶液(18∶15∶67),流速0.3 ml/min,柱温30 ℃。 结果 虎杖苷的线性范围为1.0~5 000.0 ng/ml (r=0.998 4),最低定量检测浓度为1.0

English Abstract

丁雪鹰, 侯晓丽, 孙铭学, 肖凯. LC-MS/MS测定大鼠血浆中虎杖苷浓度[J]. 药学实践与服务, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012
引用本文: 丁雪鹰, 侯晓丽, 孙铭学, 肖凯. LC-MS/MS测定大鼠血浆中虎杖苷浓度[J]. 药学实践与服务, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012
shu
DING Xue-ying, HOU Xiao-li, SUN Ming-xue, XIAO Kai. Determination of polydatin in rats plasma by LC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012
Citation: DING Xue-ying, HOU Xiao-li, SUN Ming-xue, XIAO Kai. Determination of polydatin in rats plasma by LC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012
shu

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